Document Detail


A rapid and specific assay for the determination of lamotrigine in human plasma by normal-phase HPLC.
MedLine Citation:
PMID:  9029758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and specific high-performance liquid chromatography assay of lamotrigine in human plasma is described. Lamotrigine is extracted with dichloromethane from buffered plasma to which an internal standard has been added. The solvent is directly injected into a 250 x 4.6-mm Spherisorb Silica column and the drug is eluted by using a mixture of methanol, n-heptane, dichloromethane, and 28-30% ammonium hydroxide (20:40:40:0.3 vol/vol) at a flow rate of 1 ml/min. The eluates are detected at 240 nm. The assay requires 250 microliters of sample, and concentrations as low as 0.4 microgram/ml can be measured accurately. The method is linear in the range of 0.4-16 micrograms/ml, with a mean coefficient of correlation (r) > or = 0.997. Within- and between-day relative standard deviations at three different concentration levels (1, 4, and 8 micrograms/ml) are < or = 8.6%.
Authors:
A Bartoli; R Marchiselli; G Gatti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Therapeutic drug monitoring     Volume:  19     ISSN:  0163-4356     ISO Abbreviation:  Ther Drug Monit     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-17     Completed Date:  1997-04-17     Revised Date:  2013-08-21    
Medline Journal Info:
Nlm Unique ID:  7909660     Medline TA:  Ther Drug Monit     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  100-7     Citation Subset:  IM    
Affiliation:
Clinical Pharmacology Unit, University of Pavia, Italy.
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MeSH Terms
Descriptor/Qualifier:
Anticonvulsants / blood*
Chromatography, High Pressure Liquid
Humans
Reproducibility of Results
Sensitivity and Specificity
Triazines / blood*
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Triazines; U3H27498KS/lamotrigine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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