Document Detail


A rapid, simple method for calculating equilibrium constants and antibody site concentrations from dilution curves alone.
MedLine Citation:
PMID:  8359824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid new practical method for calculating both the antibody-antigen equilibrium constant and the antibody concentration from antibody dilution curve data alone is described. This method is faster than the inhibition curve method for evaluating a humoral immune response. It is particularly suitable for monitoring the immune response of an immunization program. The response is assessed as an immunization index, Abi*Ka. This index is more exact than the antibody titer obtained from dilution curves and independent of the specific activity of the labelled molecule and total activity used in the assay. The method was used to monitor the production of a monoclonal antibody to the sulphide peptide leucotriene including immunization, cloning and purification.
Authors:
B Boutten; M Baud; M H Vicentelli; F Dray; S Mamas
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Hybridoma     Volume:  12     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-09-28     Completed Date:  1993-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Affiliation:
Laboratoire de Prédéveloppement des Réactifs Immunologiques, Institut Pasteur, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Animals
Antibody Affinity
Antigen-Antibody Reactions*
Binding Sites, Antibody
Female
Immune Sera
Kinetics
Mice
Mice, Inbred BALB C / immunology
Protein Binding
SRS-A / immunology
Chemical
Reg. No./Substance:
0/Binding Sites, Antibody; 0/Immune Sera; 0/SRS-A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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