Document Detail

A rapid and sensitive method for the determination of gamma-hydroxybutyric acid and trans-gamma-hydroxycrotonic acid in rat brain tissue by gas chromatography/mass spectrometry with negative ion detection.
MedLine Citation:
PMID:  3408819     Owner:  NLM     Status:  MEDLINE    
gamma-Hydroxybutyric acid (GHB) and trans-gamma-hydroxycrotonic acid (HCA) together with their respective internal standards were derivatized to give the pentafluorobenzyl esters of the N-tert-butyldimethylsilyl derivatives. These compounds give, under electron capture conditions, very simple negative ion mass spectra. A very sensitive and specific assay for GHB and HCA in brain tissue (detection limit of about 5 pg per injection) using gas chromatography/negative ion mass spectrometry is described. The average levels measured in the whole brain were 1.10 +/- 0.18 nmol GHB/g wet weight and 0.18 +/- 0.02 nmol HCA/g wet weight.
J D Ehrhardt; P Vayer; M Maitre
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical & environmental mass spectrometry     Volume:  15     ISSN:  0887-6134     ISO Abbreviation:  Biomed. Environ. Mass Spectrom.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-10-11     Completed Date:  1988-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603224     Medline TA:  Biomed Environ Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  521-4     Citation Subset:  IM    
Spectrometrie de Masse, Institut de Pharmacologie (UA 589 CNRS), Strasbourg, France.
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MeSH Terms
Brain Chemistry*
Gas Chromatography-Mass Spectrometry
Hydroxybutyrates / analysis*
Rats, Inbred Strains
Sodium Oxybate / analysis*
Reg. No./Substance:
0/Hydroxybutyrates; 4013-24-5/4-hydroxy-2-butenoic acid; 502-85-2/Sodium Oxybate

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