Document Detail


A rapid and sensitive method for the determination of the amount of theophylline in blood spots.
MedLine Citation:
PMID:  11291759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine the amount of theophylline in blood spots. The lowest level of theophylline analysed in a blood spot was 15 ng extracted into 250 microL and this was still considerably above the limit of quantification (3 ng in 250 microL). The levels of theophylline in blood spots correlated well with theophylline levels in plasma samples obtained from the same patients. The assay might be of use in therapeutic drug monitoring of theophylline and blood spot sampling could be applied to other drugs where therapeutic monitoring is required.
Authors:
D G Watson; E J Oliveira; A C Boyter; K D Dagg
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  53     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-04-06     Completed Date:  2001-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  413-6     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, SIBS, University of Strathclyde, Glasgow, UK. D.G.Watson@strath.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Bronchodilator Agents / blood*
Chromatography, Liquid
Female
Humans
Isotope Labeling
Male
Mass Spectrometry
Middle Aged
Reference Standards
Theophylline / blood*
Chemical
Reg. No./Substance:
0/Bronchodilator Agents; 58-55-9/Theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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