| A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B1 in food samples. | |
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MedLine Citation:
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PMID: 17723748 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibody with enhanced chemiluminescent (ECL) detection of fumonisin B1 (FB1) in food samples has been developed. Assay conditions, including concentrations of antibody and enzyme conjugate, competition time and so on, were optimized. The effects of pH and two different organic solvents were investigated. The optimized ECL-ELISA system allowed FB1 determination in a linear working range of 0.14-0.9 microg L(-1) with IC50 value of 0.32 microg L(-1) and a limit of detection of 0.09 microg L(-1). The ECL-ELISA was about 10 times more sensitive and about 30% time less than that of colorimetric ELISA using the same antibody and HRP-conjugate. Good recoveries with spiked food samples were obtained, and the results correlated well with those obtained using conventional direct competition ELISA assay and HPLC method, which indicated that ECL-ELISA was capable of being applied for the specific detection and routine monitoring of FB1 in food samples. |
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Authors:
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Ying Quan; Yan Zhang; Shuo Wang; Nanju Lee; Ivan R Kennedy |
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Publication Detail:
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Type: Journal Article Date: 2006-08-01 |
Journal Detail:
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Title: Analytica chimica acta Volume: 580 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2007-08-28 Completed Date: 2007-08-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: - |
Affiliation:
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Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnolgy, Tianjin University of Science and Technology, Tianjin 300457, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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