Document Detail


A rapid and sensitive assay for neuraminidase using peanut lectin hemagglutination: application to Vibrio cholera and Trypanosoma cruzi.
MedLine Citation:
PMID:  6352815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A neuraminidase assay based on peanut lectin agglutination is described. Human red blood cells are used both as substrate for the enzyme and as probe for the lectin. The validity of the method is ascertained by measuring the enzyme and lectin activities on erythrocytes whose outer membrane sialic acid was labeled with tritium after oxidation with sodium periodate followed by reduction with sodium borotritiide. The neuraminidases of Trypanosoma cruzi and Vibrio cholera are used as examples; in both cases, a linear relationship is observed between the degree of erythrocyte desialylation and the peanut hemagglutination titer. For the hemagglutination assay, lectin in homogeneous form as well as in crude peanut extracts may be used, and free sialic acid need not be separated from substrate-bound sialic acid. The hemagglutinating activity of peanut lectin is not affected by pre-treatment of the erythrocytes with various proteases. The method is particularly useful in the neuraminidase analysis of multiple samples, such as in gel filtration chromatography and for screening of hybridoma antibodies against neuraminidase.
Authors:
M E Pereira
Related Documents :
1060425 - Heterogeneity of carcinoembryonic antigen. ii. monosaccharide composition of concanaval...
3545325 - Toxic effect of isolated glycophorin a on the in vitro growth of plasmodium falciparum.
487365 - Spin-labelling of sialic acid in soluble and cell-surface glycoproteins.
7894145 - Variety of sialic acids occurring in the bovine sublingual gland.
7255285 - Pharmacological and metabolic studies on amino acid benzocaine derivatives.
15801715 - In vitro transcutaneous delivery of ketoprofen and essential polyunsaturated fatty acid...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  63     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-34     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel
Hemagglutination Tests / methods*
Humans
Lectins / diagnostic use*
Neuraminidase / analysis*,  metabolism
Peanut Agglutinin
Trypanosoma cruzi / enzymology*
Vibrio cholerae / enzymology*
Grant Support
ID/Acronym/Agency:
1-RO1-AI-18102-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Lectins; 0/Peanut Agglutinin; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of three tests for measuring footpad swelling in the mouse.
Next Document:  Use of human antibodies to identify antigens in cultured human tumor cells: detection of discrete an...