Document Detail


Rapid screening method for compounds that affect the growth and germination of Candida albicans, using a real-time PCR thermocycler.
MedLine Citation:
PMID:  21926199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We propose a screening method for compounds affecting growth and germination in Candida albicans using a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using P(ACT1)-GFP and P(HWP1)-GFP reporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.
Authors:
Lucja M Jarosz; Bastiaan P Krom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-16
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-03     Completed Date:  2012-02-21     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8193-6     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Antifungal Agents / pharmacology*
Candida albicans / drug effects*,  growth & development
Drug Evaluation, Preclinical / methods*
Genes, Reporter
Green Fluorescent Proteins / genetics,  metabolism
Hyphae / drug effects,  growth & development
Real-Time Polymerase Chain Reaction / methods*
Staining and Labeling / methods
Chemical
Reg. No./Substance:
0/Antifungal Agents; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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