Document Detail

A rapid screening method for Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type 30 and its related clone using a combination of multiplex PCR and pulsed-field gel electrophoresis.
MedLine Citation:
PMID:  19396516     Owner:  NLM     Status:  MEDLINE    
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), which is often positive for Panton-Valentine leucocidin (PVL), is increasingly noted as an emerging pathogen worldwide. In Japan, PVL-positive CA-MRSA belonging to multilocus sequence type (ST) 30 has spread and caused, for example, pediatric death due to community-acquired pneumonia and severe pelvic abscesses in an athlete. In this study, we investigated a new rapid screening method for PVL-positive ST30 CA-MRSA and its related clone by a combination of multiplex polymerase chain reaction (M-PCR) and pulsed-field gel electrophoresis (PFGE). For M-PCR, the targets of the assay were the five genes for PVL, collagen adhesin, bone sialoprotein adhesin, methicillin resistance, and S. aureus-specific thermostable nuclease. Only PVL-positive ST30 CA-MRSA strains produced all five bands in M-PCR. With PFGE, Japanese strains and most foreign strains of PVL-positive ST30 CA-MRSA shared the same pattern. Moreover, PFGE distinguished current PVL-positive CA-MRSA ST30/spa19 strains from previous PVL-positive MRSA ST30/spa43 strains (which were isolated at the time of nosocomial MRSA outbreaks in the late 1980s and early 1990s) in Japan. Thus, the M-PCR assay rapidly, and the M-PCR/PFGE combination assay more precisely, discriminated between PVL-positive ST30 CA-MRSA (or its related clone) and PVL-positive CA-MRSA belonging to other ST types such as ST1, 8, 59, and 80, PVL-negative CA-MRSA, hospital-acquired MRSA, methicillin-susceptible S. aureus, or coagulase-negative staphylococci (CNS), including MRCNS. This screening method is more useful than genotyping for routine work in many clinical laboratories.
Ivan Reva; Wataru Higuchi; Tomomi Takano; Olga Singur; Kyoko Ozaki; Hirokazu Isobe; Shizuka Yabe; Kohei Saito; Tatiana Baranovich; Symaa Enany; Taketo Otsuka; Vladimir Potapov; Akihito Nishiyama; Tatsuo Yamamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-25
Journal Detail:
Title:  Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy     Volume:  15     ISSN:  1341-321X     ISO Abbreviation:  J. Infect. Chemother.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-27     Completed Date:  2009-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9608375     Medline TA:  J Infect Chemother     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  75-83     Citation Subset:  IM    
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, 757 Ichibanchou, Asahimachidori, Niigata, 951-8510, Japan.
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MeSH Terms
Bacterial Proteins / genetics
Bacterial Toxins / genetics*
Community-Acquired Infections / microbiology*
Cross Infection / microbiology
Electrophoresis, Gel, Pulsed-Field / methods*
Exotoxins / genetics*
Leukocidins / genetics*
Methicillin-Resistant Staphylococcus aureus / classification,  genetics,  isolation & purification*
Polymerase Chain Reaction / methods*
Staphylococcal Infections / microbiology*
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Exotoxins; 0/Leukocidins; 0/Panton-Valentine leukocidin

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