Document Detail


A rapid and precise assay for peroxide as 'active oxygen' in products, by flow injection analysis in a high pressure system with spectrophotometric detection.
MedLine Citation:
PMID:  18969726     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A simple, rapid and automated assay for 'active oxygen' originating from hydrogen peroxide, or other organic peroxides, in products is presented employing flow injection (FI) analysis. The product is dispersed and peroxide dissolved in a solvent of 5% (v/v) acetic acid, which constitutes the carrier stream. Ammonium molybdate can be added to this carrier stream to increase sensitivity as required. The sample solution is injected into the acid carrier stream, which is then merged with iodide ion in situ in a two-stream manifold. The 'active oxygen' in the product oxidises acidified iodide to iodine, which is detected spectrophotometrically at 350nm. The closed conditions prevent interference from atmospheric oxygen and the short reaction time minimises the potential for interference from side reactions. Standard HPLC equipment is used throughout, employing a back-pressure to improve precision (high pressure flow injection). Conditions have been investigated using screening multivariate experimental design (two-level quarter fractional factorial design incorporating centre points) to identify and optimise the critical variables. The method has been fully validated (with sample solution R.S.D.s typically < 0.5%, LOQs of 0.04 or 0.006mugml(-1) as 'active oxygen' for acid or acid/molybdate carriers respectively) and is quicker and simpler than the currently employed manual titration approach. It should be applicable to a range of 'active oxygen' products.
Authors:
M S Bloomfield
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  64     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1175-82     Citation Subset:  -    
Affiliation:
GlaxoSmithkline Consumer Healthcare, St Georges Avenue, Weybridge, Surrey KT13 0DE, UK.
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