| A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food. | |
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MedLine Citation:
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PMID: 20188974 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid surface plasmon resonance (SPR) screening assay has been developed for the combined detection of T-2 and HT-2 toxins in naturally contaminated cereals using a sensor chip coated with an HT-2 toxin derivative and a monoclonal antibody. The antibody raised against HT-2 displayed high cross-reactivity with T-2 toxin while there was no cross-reaction observed with other commonly occurring trichothecenes. A simple extraction procedure using 40% methanol was applied to baby food, breakfast cereal, and wheat samples prior to biosensor analysis. Limits of detection (LOD) for each matrix were determined as 25 microg kg(-1) for baby food and breakfast cereal and 26 microg kg(-1) for wheat. Intra-assay precision (n=6) was calculated for each matrix. The results were expressed as the relative standard deviation and determined as 2.8% (100 microg kg(-1)) and 1.8% (200 microg kg(-1)) in breakfast cereal, 4.6% (50 microg kg(-1)) and 3.6% (100 microg kg(-1)) in wheat and 0.97% (25 microg kg(-1)) and 6.3% (50 microg kg(-1)) in baby food. Between run precision (n=3) performed at the same levels yielded relative standard deviations of 6.7% and 3.9% for breakfast cereals, 3.3% and 1.6% for wheat and 6.8% and 0.08% for baby food, respectively. |
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Authors:
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Julie P Meneely; Michael Sulyok; Sabine Baumgartner; Rudolf Krska; Christopher T Elliott |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-11 |
Journal Detail:
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Title: Talanta Volume: 81 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 630-6 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute of Agri-food and Land Use, School of Biological Sciences, Queen's University of Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. j.p.meneely@qub.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cereals
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chemistry* Food Analysis Immunoassay / methods* Infant Food / analysis* Optical Processes* Reproducibility of Results T-2 Toxin / analogs & derivatives*, analysis*, immunology Time Factors Zea mays / chemistry* |
| Chemical | |
Reg. No./Substance:
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21259-20-1/T-2 Toxin; 26934-87-2/HT-2 toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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