Document Detail


A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food.
MedLine Citation:
PMID:  20188974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid surface plasmon resonance (SPR) screening assay has been developed for the combined detection of T-2 and HT-2 toxins in naturally contaminated cereals using a sensor chip coated with an HT-2 toxin derivative and a monoclonal antibody. The antibody raised against HT-2 displayed high cross-reactivity with T-2 toxin while there was no cross-reaction observed with other commonly occurring trichothecenes. A simple extraction procedure using 40% methanol was applied to baby food, breakfast cereal, and wheat samples prior to biosensor analysis. Limits of detection (LOD) for each matrix were determined as 25 microg kg(-1) for baby food and breakfast cereal and 26 microg kg(-1) for wheat. Intra-assay precision (n=6) was calculated for each matrix. The results were expressed as the relative standard deviation and determined as 2.8% (100 microg kg(-1)) and 1.8% (200 microg kg(-1)) in breakfast cereal, 4.6% (50 microg kg(-1)) and 3.6% (100 microg kg(-1)) in wheat and 0.97% (25 microg kg(-1)) and 6.3% (50 microg kg(-1)) in baby food. Between run precision (n=3) performed at the same levels yielded relative standard deviations of 6.7% and 3.9% for breakfast cereals, 3.3% and 1.6% for wheat and 6.8% and 0.08% for baby food, respectively.
Authors:
Julie P Meneely; Michael Sulyok; Sabine Baumgartner; Rudolf Krska; Christopher T Elliott
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-11
Journal Detail:
Title:  Talanta     Volume:  81     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  630-6     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier B.V. All rights reserved.
Affiliation:
Institute of Agri-food and Land Use, School of Biological Sciences, Queen's University of Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. j.p.meneely@qub.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Cereals / chemistry*
Food Analysis
Immunoassay / methods*
Infant Food / analysis*
Optical Processes*
Reproducibility of Results
T-2 Toxin / analogs & derivatives*,  analysis*,  immunology
Time Factors
Zea mays / chemistry*
Chemical
Reg. No./Substance:
21259-20-1/T-2 Toxin; 26934-87-2/HT-2 toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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