Document Detail


A rapid method for the measurement of estradiol and hydrocortisone levels in maternal and fetal blood and amniotic fluid.
MedLine Citation:
PMID:  1115113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estradiol and hydrocortisone levels in maternal and fetal (umbilical cord) blood in human pregnancy can be simultaneously assessed by this method. Chromatography on microcolumns of Sephadex LH-20 with benzene:methanol (85:15) as developing solvent is used to separate estradiol, hydrocortisone, and isatin (a yellow-colored substance used as marker) from interfering steroids. Human late pregnancy plasma is used as the source of both sex hormone-binding globulin and transcortin for the competitive protein-binding assay of estradiol and hydrocortisone, respectively. The method is rapid enough to permit the monitoring of a high-risk pregnancy for plasma estradiol levels on a daily basis. The method is sensitive enough to permit assessment of perinatal adrenocortical function. Hydrocortisone levels can be measured in as little as 3 ml. of amniotic fluid obtained by amniocentesis and preliminary results indicated that hydrocortisone can be determined in as little as 0.1 ml. of fetal scalp blood or newborn baby blood.
Authors:
S Sybulski; G B Maughan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  121     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-05-10     Completed Date:  1975-05-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  32-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / analysis*
Benzene / diagnostic use
Binding Sites
Binding, Competitive
Blood Specimen Collection
Chromatography / instrumentation,  methods
Estradiol / analysis*,  blood
Estriol / blood
Estrone / blood
Female
Fetus / metabolism*
Humans
Hydrocortisone / analysis*,  blood
Methanol / diagnostic use
Pregnancy
Protein Binding
Solvents
Testosterone / blood
Chemical
Reg. No./Substance:
0/Solvents; 50-23-7/Hydrocortisone; 50-27-1/Estriol; 50-28-2/Estradiol; 53-16-7/Estrone; 58-22-0/Testosterone; 67-56-1/Methanol; 71-43-2/Benzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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