Document Detail

A rapid method for isolation of retinal pigment epithelial cells from rat eyeballs.
MedLine Citation:
PMID:  8552366     Owner:  NLM     Status:  MEDLINE    
A new, simple method for isolating retinal pigment epithelial (RPE) cells from rat eyeballs was developed: incubation of the eyeballs in 0.1% proteinase K solution. This method yielded an average of 4 x 10(4) viable RPE cells from each Sprague-Dawley rat eyeball in 1 h. These RPE cells were hexagonal and had cytokeratin in their cytoplasm and numerous microvilli on their surface, similar to RPE cells evaluated in vivo. The results of this study thus show that our method for incubating eyeballs in proteinase K can provide good-quality RPE cells in sufficient quantities for study in a short time.
K Sakagami; H Naka; A Hayashi; M Kamei; T Sasabe; Y Tano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ophthalmic research     Volume:  27     ISSN:  0030-3747     ISO Abbreviation:  Ophthalmic Res.     Publication Date:  1995  
Date Detail:
Created Date:  1996-02-20     Completed Date:  1996-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0267442     Medline TA:  Ophthalmic Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  262-7     Citation Subset:  IM    
Department of Ophthalmology, Ehime University School of Medicine, Japan.
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MeSH Terms
Cell Division
Cell Separation / methods*
Cell Survival
Endopeptidase K
Eye / drug effects
Fluorescent Antibody Technique, Indirect
Glial Fibrillary Acidic Protein / analysis
Immunoenzyme Techniques
Keratins / analysis
Microscopy, Electron, Scanning
Pigment Epithelium of Eye / chemistry,  cytology*,  ultrastructure
Rats, Sprague-Dawley
Serine Endopeptidases / pharmacology
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 68238-35-7/Keratins; EC 3.4.21.-/Serine Endopeptidases; EC K

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