Document Detail


A rapid method for identification of Mycobacterium species by polyacrylamide gel electrophoresis of soluble cell proteins.
MedLine Citation:
PMID:  1990134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of an easily and rapidly prepared soluble protein fraction were used in conjunction with conventional techniques to identify different strains of Mycobacterium tuberculosis, M. bovis, M. bovis BCG, M. africanum, M. avium, M. kansasii, M. marinum, M. gastri, M. simiae and M. malmoense. Complete concordance of results from both methods was obtained with all species except those of the M. tuberculosis complex. With the SDS-PAGE technique, all strains of the M. tuberculosis complex were recognised as belonging to one species. By visual analysis of the SDS-PAGE polypeptide profiles, only minor differences between strains of the same species were seen and each species showed a characteristic polypeptide profile. Quantitation of the data by calculation of the Dice coefficient of similarity of the band positions obtained by densitometry indicated that the similarity between different strains of one species was 90-100% and the similarity between the species was in the range 30-45%. The results indicate that SDS-PAGE is a simple and rapid method for identifying mycobacterial strains.
Authors:
A De Jong; A H Hoentjen; A G Van Der Zanden
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  34     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-03-07     Completed Date:  1991-03-07     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
Affiliation:
Regional Institute for Public Health, Deventer, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis*
Densitometry
Electrophoresis, Polyacrylamide Gel*
Mycobacterium / isolation & purification*
Solubility
Species Specificity
Streptococcus pyogenes / analysis
Chemical
Reg. No./Substance:
0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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