Document Detail

A rapid method for the determination of deoxycytidylate deaminase activity in pregnancy serum.
MedLine Citation:
PMID:  240523     Owner:  NLM     Status:  MEDLINE    
A method is described for determining the activity of deoxycytidylate deaminase in serum. The ammonia liberated from deoxycytidine monophosphate has been specifically determined by enzymatic amination of alpha-ketoglutarate using glutamate dehydrogenase. The concurrent oxidation of NADH2 at 340 nm was proportional to the ammonia liberated from deoxycytidine monophosphate. Using the technique described, a result would be available to the clinician in under 4.5 h. The "normal activity" for deoxycytidine deaminase in normal male, female and pregnancy sera has been determined.
L Targett-Adams; D D Jones; G F Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  63     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1975 Sep 
Date Detail:
Created Date:  1975-12-28     Completed Date:  1975-12-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  377-82     Citation Subset:  IM    
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MeSH Terms
DCMP Deaminase / blood*
Evaluation Studies as Topic
Fetal Blood / enzymology
Glutamate Dehydrogenase / blood
Hydrogen-Ion Concentration
Nucleotide Deaminases / blood*
Pregnancy Trimester, Second
Pregnancy Trimester, Third
Spectrophotometry, Ultraviolet / methods
Time Factors
Reg. No./Substance:
EC Dehydrogenase; EC 3.5.4.-/Nucleotide Deaminases; EC Deaminase

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