| A rapid method for the determination of deoxycytidylate deaminase activity in pregnancy serum. | |
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MedLine Citation:
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PMID: 240523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described for determining the activity of deoxycytidylate deaminase in serum. The ammonia liberated from deoxycytidine monophosphate has been specifically determined by enzymatic amination of alpha-ketoglutarate using glutamate dehydrogenase. The concurrent oxidation of NADH2 at 340 nm was proportional to the ammonia liberated from deoxycytidine monophosphate. Using the technique described, a result would be available to the clinician in under 4.5 h. The "normal activity" for deoxycytidine deaminase in normal male, female and pregnancy sera has been determined. |
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Authors:
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L Targett-Adams; D D Jones; G F Williams |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 63 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1975 Sep |
Date Detail:
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Created Date: 1975-12-28 Completed Date: 1975-12-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 377-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DCMP Deaminase
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blood* Evaluation Studies as Topic Female Fetal Blood / enzymology Glutamate Dehydrogenase / blood Humans Hydrogen-Ion Concentration Kinetics Male Nucleotide Deaminases / blood* Pregnancy* Pregnancy Trimester, Second Pregnancy Trimester, Third Spectrophotometry, Ultraviolet / methods Time Factors |
| Chemical | |
Reg. No./Substance:
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EC 1.4.1.2/Glutamate Dehydrogenase; EC 3.5.4.-/Nucleotide Deaminases; EC 3.5.4.12/DCMP Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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