Document Detail


A rapid method for the detection of circulating immune complexes in biological fluids by counterimmunoelectrophoresis.
MedLine Citation:
PMID:  7123115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Owing to their great content in neuraminic acid and/or nucleic acids, most of the circulating immune complexes run significantly faster as unbound gammaglobulins during agar gel electrophoresis. Therefore, because of their immunoglobulin components they can be easily detected in the alpha-1, inter-alpha, alpha-2 and beta fractions with monospecific anti-Ig-sera by counterimmunoelectrophoresis. The presence of Factor III of complement and of fibrinogen, in some cryoglobulins can be proved in the some way. Finally, nucleic acid content (both RNA and DNA), and presence of neuraminic acid in precipitated immune complexes can be demonstrated by usual histologic methods (Giemsa, methyl green-pyronine, PAS or Aminoff) on washed and dried slides.
Authors:
E J Módy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Médecine interne     Volume:  20     ISSN:  0377-1202     ISO Abbreviation:  Med Interne     Publication Date:    1982 Apr-Jun
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7506353     Medline TA:  Med Interne     Country:  ROMANIA    
Other Details:
Languages:  eng     Pagination:  165-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigen-Antibody Complex / analysis*
Counterimmunoelectrophoresis*
Immunoelectrophoresis*
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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