| A rapid method for detection of N-acetylglucosaminidase-type chitinase activity in crossed immunoelectrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels using 4-methylumbelliferyl-N-acetyl-D-glucosaminide as substrate. | |
| | |
MedLine Citation:
|
PMID: 7925246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A method for the detection of N-acetylglucosaminidase (GlcNAcase) activity has been developed by using 4-methyl-umbelliferyl-N-acetyl-D-glucosaminide (4-MU-GlcNAc) as substrate in crossed immunoelectrophoresis (CIE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Visualization of the reaction with a 366 nm ultra-violet light is possible in approximately 30 min. The method is fast and sensitive in comparison with previous methods. The same band as in SDS-PAGE, showing both GlcNAcase and chitinase activity, was found in the present study; we therefore conclude that this method is also useful in a GlcNAcase-type chitinase assay. |
| | |
Authors:
|
K S Chen; K K Lee; H C Chen |
Related Documents
:
|
6433926 - Purification of c-reactive protein. 298186 - Polyploidy, plants, and electrophoresis. 7650756 - Chromatographic and immunochemical characterization of rat brain neuropeptide y-like im... 18428306 - Detection of mutations by denaturing gradient gel electrophoresis. 23424016 - Selective recognition of triamterene in biological samples by molecularly imprinted mon... 11334356 - Liquid chromatographic-mass spectrometric determination of celecoxib in plasma using si... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Electrophoresis Volume: 15 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 1994 May |
Date Detail:
|
Created Date: 1994-10-26 Completed Date: 1994-10-26 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 662-5 Citation Subset: IM |
Affiliation:
|
Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylglucosamine
/
analogs & derivatives* Acetylglucosaminidase / analysis* Chitinase / analysis* Electrophoresis, Polyacrylamide Gel / instrumentation* Fluorescence Gels* Hymecromone / analogs & derivatives* Immunoelectrophoresis, Two-Dimensional / instrumentation* Sensitivity and Specificity Substrate Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Gels; 37067-30-4/4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside; 7512-17-6/Acetylglucosamine; 90-33-5/Hymecromone; EC 3.2.1.14/Chitinase; EC 3.2.1.52/Acetylglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An examination of the beta-glucosidase (linamarase) banding pattern in flax seedlings using Ferguson...
Next Document: Studies of serum protein complexes with nickel using crossed immunoelectrophoresis.