Document Detail

A rapid method of Sertoli cell isolation by DSA lectin, allowing mitotic analyses.
MedLine Citation:
PMID:  10022769     Owner:  NLM     Status:  MEDLINE    
We have developed a rapid and convenient method of Sertoli cell preparation for studying the growth kinetics of these cells in in vitro culture. Datura Stramonium agglutinin (DSA)-coated dishes were used to rapidly purify single Sertoli cells from immature rat testis. We have monitored by immunohistochemical markers the degree of contamination of our Sertoli cell preparation by other cell types. The cell preparation is essentially free of germ cells and interstitial cells and contains a minimal percentage of myoid cells. Sertoli cells isolated with this method retain functional activities such as the FSH responsiveness in terms of cAMP production. In addition, we have studied the proliferative activity of Sertoli cells isolated by lectin binding from rats of different ages. Sertoli cells exhibited a characteristic pattern of proliferation which was a function of the donor animal age. The proliferative activity of isolated Sertoli cells decreased with age, being much higher in 3 day-old rats than in older animals. A similar pattern was observed when the mitotic activity of Sertoli cells in response to mitogens present in the testicular extracts from 5 day-old rats was evaluated. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.
S Scarpino; A R Morena; C Petersen; B Fröysa; O Söder; C Boitani
Related Documents :
2053849 - Comparative study on metallothionein induction in whole testicular tissue and isolated ...
15206959 - Expression and function of class b scavenger receptor type i on both apical and basolat...
20586549 - Sertoli-leydig cell tumour in a 13-year-old girl.
15716129 - Rat sertoli cells express epithelial but also mesenchymal genes after immortalization w...
163729 - Rat sertoli cells: a rapid method for obtaining viable cells.
9569679 - The effect of chronic luteinizing hormone treatment on adult rat leydig cells.
18494609 - Effects of (15s)-hydroperoxyeicosatetraenoic acid and (15s)-hydroxyeicosatetraenoic aci...
15162459 - Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in ...
19085139 - Microinjection of follicle-enclosed mouse oocytes.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  146     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  121-7     Citation Subset:  IM    
Department of Histology and Medical Embryology, University of Rome La Sapienza, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Division
Cell Separation / methods*
Cyclic AMP / biosynthesis
DNA / biosynthesis
Follicle Stimulating Hormone / pharmacology
Keratins / analysis
Plant Lectins*
Rats, Wistar
Sertoli Cells / cytology*,  drug effects,  metabolism
Testis / cytology,  growth & development
Reg. No./Substance:
0/Datura stramonium lectin; 0/Lectins; 0/Plant Lectins; 60-92-4/Cyclic AMP; 68238-35-7/Keratins; 9002-68-0/Follicle Stimulating Hormone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter...
Next Document:  Effects of ACTH administration on zonation of the guinea pig adrenal cortex.