Document Detail


A rapid method of Sertoli cell isolation by DSA lectin, allowing mitotic analyses.
MedLine Citation:
PMID:  10022769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a rapid and convenient method of Sertoli cell preparation for studying the growth kinetics of these cells in in vitro culture. Datura Stramonium agglutinin (DSA)-coated dishes were used to rapidly purify single Sertoli cells from immature rat testis. We have monitored by immunohistochemical markers the degree of contamination of our Sertoli cell preparation by other cell types. The cell preparation is essentially free of germ cells and interstitial cells and contains a minimal percentage of myoid cells. Sertoli cells isolated with this method retain functional activities such as the FSH responsiveness in terms of cAMP production. In addition, we have studied the proliferative activity of Sertoli cells isolated by lectin binding from rats of different ages. Sertoli cells exhibited a characteristic pattern of proliferation which was a function of the donor animal age. The proliferative activity of isolated Sertoli cells decreased with age, being much higher in 3 day-old rats than in older animals. A similar pattern was observed when the mitotic activity of Sertoli cells in response to mitogens present in the testicular extracts from 5 day-old rats was evaluated. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.
Authors:
S Scarpino; A R Morena; C Petersen; B Fröysa; O Söder; C Boitani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  146     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  121-7     Citation Subset:  IM    
Affiliation:
Department of Histology and Medical Embryology, University of Rome La Sapienza, Italy.
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Cell Division
Cell Separation / methods*
Cyclic AMP / biosynthesis
DNA / biosynthesis
Follicle Stimulating Hormone / pharmacology
Keratins / analysis
Lectins*
Male
Mitosis*
Plant Lectins*
Rats
Rats, Wistar
Sertoli Cells / cytology*,  drug effects,  metabolism
Testis / cytology,  growth & development
Chemical
Reg. No./Substance:
0/Datura stramonium lectin; 0/Lectins; 0/Plant Lectins; 60-92-4/Cyclic AMP; 68238-35-7/Keratins; 9002-68-0/Follicle Stimulating Hormone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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