Document Detail


A rapid lateral flow test for the determination of total type B fumonisins in maize.
MedLine Citation:
PMID:  19756539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A one-step lateral flow test was developed for the quantitative determination of total type B fumonisins in maize with a test range up to 4,000 microg/kg and a limit of detection of 199 microg/kg. The test presented gives a result within 4 min, including 1 min strip drying, and does not require any sample cleanup steps after a previous 3-min sample extraction. Quantitative readout with a compact photometric strip reader will also indicate the best suited measurement range when needed. The test is based on a competitive immunoassay format where a ready-to-use antibody-colloidal gold particle complex is mixed with 50 microL sample extract in a microwell and used as a signal reagent. The test strip is inserted into the well and the mixed content migrates onto the strip, which contains a test zone and a control zone. Mycotoxin-protein conjugate coated on the test zone captures free signal reagent, and colored particles concentrate, forming a visible line. The intensity of the test line is dependent on the total fumonisin concentration in the sample. Naturally contaminated quality-control maize material was used for matrix-matched calibration of photometric readout. The test presented is both quantitative and rapid, with no cross-reactivity to other mycotoxins. The applicability of the lateral flow test was shown by the screening of 23 naturally contaminated maize samples. Relative standard deviations ranged from 1.7 to 32.9%.
Authors:
Alexandra Molinelli; Karina Grossalber; Rudolf Krska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-12
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  395     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1309-16     Citation Subset:  IM    
Affiliation:
Romer Labs Division Holding, Technopark 1, 3430 Tulln, Austria.
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MeSH Terms
Descriptor/Qualifier:
Calibration
Cross Reactions
Fumonisins / analysis*
Immunoassay / methods*
Time Factors
Zea mays / chemistry*
Chemical
Reg. No./Substance:
0/Fumonisins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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