Document Detail


The rapid isolation from human blood of concentrated, white-cell-free preparations of Plasmodium falciparum.
MedLine Citation:
PMID:  1096379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sucrose gradient centrifugation method has been applied to representative samples of human (Gambian) blood infected with ring, trophozoite, schizont and gametocyte stages of P. falciparum in order to assess quantitatively the efficiency of recovery, white cell removal and the degree of enrichment of the infected cell fraction. Maximal white cell removal was 90%. (a.v.) Average infected cell recoveries varied with the level of white cell contamination, namely 47% (2-5% WBC), 34% (1-2% WBC) and 24% (smaller than 1% WBC); infected cells were enriched 2-9-fold on average, and up to 22-fold in the case of gametocytes. Preliminary attempts to prepare free parasites by nitrogen cavitation of infected cells showed that disruption took place at much lower pressures than those required to break normal red cells. Gel diffusion analyses showed that the most highly purified infected cell preparations retained the full precipitinogenic spectrum of the original crude preparation.
Authors:
J Williamson; B Cover
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transactions of the Royal Society of Tropical Medicine and Hygiene     Volume:  69     ISSN:  0035-9203     ISO Abbreviation:  Trans. R. Soc. Trop. Med. Hyg.     Publication Date:  1975  
Date Detail:
Created Date:  1975-09-29     Completed Date:  1975-09-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7506129     Medline TA:  Trans R Soc Trop Med Hyg     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens / isolation & purification
Cell Separation / methods*
Centrifugation, Density Gradient / methods
Erythrocytes / parasitology,  ultrastructure
Gambia
Humans
Leukocytes / analysis*
Malaria / blood*,  immunology
Microscopy, Electron, Scanning
Plasmodium falciparum / isolation & purification*
Chemical
Reg. No./Substance:
0/Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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