Document Detail


The rapid incorporation of radioactive fatty acid into triacylglycerols during the in vitro acylation of native lipids of neuronal nuclei.
MedLine Citation:
PMID:  6849958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using neuronal nuclei (N1) and microsomes (P3) isolated from cerebral cortices of 15-day-old rabbits, the incorporation of [14C]oleate was followed in vitro, making use of fatty acid activation factors and endogenous membrane acyl acceptors. Of the lipids of N1, it was triacylglycerol which showed the highest rates of labelling and which represented 71-80% of the total incorporated radioactivity in this fraction. Specific rates of N1 triacylglycerol formation were 63-166 times those of P3 triacylglycerols (based upon membrane phospholipid content). In P3, phospholipids made up 85% of the total microsomal lipid labelling. The incorporation of oleate was dependent upon ATP and coenzyme A, and acyl-CoA synthetase activities were demonstrated in N1 and P3 (specific activity ratio, N1:P3 = 4.5). Using exogenous [14C]oleoyl-CoA, high rates of N1 triacylglycerol labelling were still seen relative to P3, but rates of diacylglycerol and phospholipid labelling were substantially elevated in both fractions in contrast to rates found using [14C]oleate. By increasing levels of endogenous diacylglycerol using preincubations with phospholipase C, a 3-fold increase was seen in specific rates of triacylglycerol formation in both fractions in subsequent assays with [14C]oleate. A 4.5-fold increase in N1 diacylglycerol concentrations was found when N1 was incubated for 10 min in the absence of fatty acid, ATP and coenzyme A. It is concluded that neuronal nuclei have a very active diacylglycerol acyltransferase as well as the ability to generate diacylglycerol substrates.
Authors:
R R Baker; H Y Chang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  752     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-15     Completed Date:  1983-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acylation
Animals
Carbon Radioisotopes
Cell Nucleus / metabolism*
Cerebral Cortex / metabolism*
Isotope Labeling
Male
Membrane Lipids / metabolism
Microsomes / metabolism
Neurons / metabolism*
Oleic Acid
Oleic Acids / metabolism*
Phospholipids / metabolism
Rabbits
Triglycerides / biosynthesis*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Membrane Lipids; 0/Oleic Acids; 0/Phospholipids; 0/Triglycerides; 112-80-1/Oleic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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