| A rapid in vitro screening system for the identification and evaluation of anticancer drugs. | |
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MedLine Citation:
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PMID: 2590857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the development of an in vitro screening system that can be used to identify new anticancer drugs that are specifically cytotoxic for dividing cells. The screening system takes advantage of the potential of many cell lines, including tumor cells, to stop dividing when they are plated at high cell density. The cytotoxic effects of anticancer drugs on dividing (i.e., cells plated at low cell density) and nondividing cells (i.e., cells plated at high cell density) is measured by the incorporation of 51Cr. This in vitro system was evaluated by measuring the cytotoxic effects of the anticancer drugs cisplatin, thiotepa, doxorubicin, methotrexate, and vinblastine on the cell lines B/C-N, ME-180, and MCF-7. In this in vitro system the concentrations of the anticancer drugs that produced significant cytotoxicity on only dividing cells are similar to the concentrations that are used clinically. The fact that this in vitro system is rapid, simple, applicable to many cell types, and able to predict effective concentrations of anticancer drugs should make it useful for the screening of new anticancer drugs and for the design of preclinical studies. |
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Authors:
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J W Kao; J L Collins |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Cancer investigation Volume: 7 ISSN: 0735-7907 ISO Abbreviation: Cancer Invest. Publication Date: 1989 |
Date Detail:
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Created Date: 1990-01-17 Completed Date: 1990-01-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8307154 Medline TA: Cancer Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 303-11 Citation Subset: IM |
Affiliation:
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Washington University School of Medicine, St. Louis, Missouri 63110. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents* / pharmacology Cell Line Cell Survival / drug effects Chromium Radioisotopes Dose-Response Relationship, Drug Drug Evaluation, Preclinical / methods* Humans Mice Thymidine / metabolism Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Chromium Radioisotopes; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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