| A rapid gel electrophoretic chip for serum cholesterol determination. | |
| | |
MedLine Citation:
|
PMID: 21390373 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We present a rapid gel electrophoretic chip, composed of 2.5% (w/v) acrylamide and 1% (w/v) agarose gel, for serum cholesterol determination using a photo lithography technique. After optimizations, we determined the lipoprotein concentration of standard serum using a conventional enzyme method. The serum was diluted, stained and loaded for 15 min onto the chip. After loading, the intensities of low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) bands separated at the chip were estimated using an image analyzer. The intensities of these bands corresponded to concentrations obtained from a standard enzyme-based method. The detected LDL-C and HDL-C concentrations were linear up to 146 mg dL(-1) and 53 mg dL(-1) respectively. Finally, we carried out the cholesterol analysis using real biological samples obtained from nine volunteers using our electrophoretic chip. The LDL-C and HDL-C levels detected using our chip correlated well with the results obtained using the conventional enzyme-based method r(2) = 0.98 and r(2) = 0.86 for LDL-C and HDL-C, respectively. Although our sample size is small and confined only to health volunteers, we have demonstrated that this proof-of-concept gel electrophoretic chip can determine lipoproteins, simultaneously. |
| | |
Authors:
|
Kenta Kaminikado; Ryuzo Ikeda; Koutaro Idegami; Naoki Nagatani; Mun'delanji C Vestergaard; Masato Saito; Eiichi Tamiya |
Related Documents
:
|
19331583 - Hindgut plasticity in wallabies fed hay either unchopped or ground and pelleted: fiber ... 101073 - Influence of diet and age on fecal bacterial enzymes. 11060963 - Incorporating soy protein into a low-fat, low-cholesterol diet. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-10 |
Journal Detail:
|
Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Voltammetric characterization of a fully integrated, patterned single walled carbon nanotube three-e...
Next Document: A permanently porous van der Waals solid by using phosphonate monoester linkers in a metal organic f...