Document Detail


A rapid gas chromatography-mass spectrometry method for quantification of alkylresorcinols in human plasma.
MedLine Citation:
PMID:  19010298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alkylresorcinols (ARs) are phenolic lipids that among foods are found almost exclusively in whole grain and bran products based on wheat and rye. They have been suggested to be used as selective biomarkers of whole grain wheat and rye intake and, thus, may serve as an alternative/complement to commonly used dietary assessment methods in epidemiological studies. For such investigations where analysis of large numbers of valuable samples is required, rapid, sensitive, and repeatable methods are essential. In this article, we describe a rapid and sensitive gas chromatography-mass spectrometry (GC-MS) method for quantification of AR homologues C17:0, C19:0, C21:0, C23:0, and C25:0 in human plasma. The method uses Oasis MAX solid phase extraction cartridges for sample cleanup. A plasma sample of 0.2 ml could be used without preincubation with water. Samples in the range of 7 to 8750 nmol total AR/L were successfully analyzed with the method described, and the average total AR recovery within the reported range was 92+/-12%. The within- and between-day precision values of total AR concentration in a quality control sample, determined as the coefficients of variation, were on average 7 and 10%, respectively. Approximately 30 to 50 samples could be analyzed within 1 day. The improved GC-MS method presented can be used for rapid analysis of ARs in relatively small sample volumes.
Authors:
Rikard Landberg; Per Man; Afaf Kamal-Eldin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-31
Journal Detail:
Title:  Analytical biochemistry     Volume:  385     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-29     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7-12     Citation Subset:  IM    
Affiliation:
Department of Food Science, Swedish University of Agriculture Science (SLU), Box 7051, SE-75007 Uppsala, Sweden. rikard.landberg@lmv.slu.se
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MeSH Terms
Descriptor/Qualifier:
Alkylation
Cohort Studies
Gas Chromatography-Mass Spectrometry / methods*
Humans
Reproducibility of Results
Resorcinols / blood*,  chemistry*
Sensitivity and Specificity
Solid Phase Extraction
Time Factors
Chemical
Reg. No./Substance:
0/Resorcinols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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