| A rapid flow cytometry assay for the assessment of calcium mobilization in human neutrophils in a small volume of lysed whole-blood. | |
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MedLine Citation:
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PMID: 19000691 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flow cytometry-based methods have been developed to measure most neutrophil responses. The assessment of the mobilization of calcium, however, is routinely performed on neutrophils isolated from whole blood. This report describes a flow cytometry-based assay to measure the mobilization of calcium in neutrophils directly in whole blood. This method requires minimal sample manipulation, small volumes of blood and is performed in a short period of time. Both clinical and research laboratories will be able to assess neutrophil function and the quality of granulocyte preparations using a more time and cost effective calcium mobilization test. |
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Authors:
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Philippe Desmeules; Maurice Dufour; Maria J G Fernandes |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-08 |
Journal Detail:
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Title: Journal of immunological methods Volume: 340 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-03-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 154-7 Citation Subset: IM |
Affiliation:
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Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHUQ-CHUL, Département d'Anatomie-Physiologie, Faculté de Médecine, Université Laval, Quebec, Canada, G1V 4G2. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Cells
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metabolism* Calcium / analysis*, metabolism* Flow Cytometry / methods* Humans Kinetics N-Formylmethionine Leucyl-Phenylalanine Time Factors Zymosan |
| Chemical | |
Reg. No./Substance:
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59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 7440-70-2/Calcium; 9010-72-4/Zymosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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