Document Detail


A rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.
MedLine Citation:
PMID:  2131168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relationship between volatile fatty acids (VFAs) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. VFAs were detected by the direct injection of pus into gas-liquid chromatography (GLC). Bacterial examination was carried out by anaerobic culture using blood agar plates. The bacterial identification was carried out mainly according to the VPI manual. Analysis of the direct VFA patterns of each sample resulted in 5 groups. The following bacterial species were the main isolates in each group: Streptococcus intermedius in Group A, Peptostreptococcus micros in Group B, Fusobacterium nucleatum in Group C, Bacteroides gingivalis in Group D, and Peptostreptococcus anaerobius in Group E. The profile of VFAs produced in the PYG culture medium of the above isolated bacteria was compared with the direct VFA patterns. Agreement ratios between direct and PYG VFA patterns were as follows: Group A, 47.1%; Groups B and C, 45.0%; Group D, 87.5%; and Group E, 62.9%. The acetic acid concentration was more than 14 x 10(-4) meq/ml in Group B, the butyric acid concentration was more than 7 x 10(-4) meq/ml in Group C, and the iso-caproic acid concentration was more than 14 x 10(-4) meq/ml in Group E. In these cases, it was found that the agreement ratios between the direct and PYG FVA pattern were high. In Group D, irrespective of the concentration of iso-valeric acid detected, the agreement ratio was very high. The antibiotic susceptibility of the isolates was studied. Efficiency rates of ABPC, PIPC, CCL, CEZ, CMZ, SBT/CPZ, JM, CLDM, MINO and GM were relatively low and resistant rates were high for the gram-negative rods.
Authors:
J I Tanaka; N Takano; H Unozawa; S Shigematsu; Y Kishino; H Yonezu; H Matsuzaki; T Shigematsu; S Takahashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Bulletin of Tokyo Dental College     Volume:  31     ISSN:  0040-8891     ISO Abbreviation:  Bull. Tokyo Dent. Coll.     Publication Date:  1990 May 
Date Detail:
Created Date:  1991-11-22     Completed Date:  1991-11-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7505414     Medline TA:  Bull Tokyo Dent Coll     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  155-62     Citation Subset:  D    
Affiliation:
Tokyo Dental College.
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MeSH Terms
Descriptor/Qualifier:
Abscess / diagnosis,  microbiology
Adolescent
Adult
Aged
Bacteria, Anaerobic / isolation & purification*
Bacterial Infections / diagnosis*
Child
Child, Preschool
Chromatography, Gas
Fatty Acids, Volatile / analysis*
Female
Humans
Male
Middle Aged
Mouth Diseases / diagnosis,  microbiology
Periodontal Abscess / diagnosis*,  microbiology*
Suppuration / microbiology
Chemical
Reg. No./Substance:
0/Fatty Acids, Volatile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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