Document Detail

A rapid densitometric method for simultaneous quantification of gallic acid and ellagic acid in herbal raw materials using HPTLC.
MedLine Citation:
PMID:  15881089     Owner:  NLM     Status:  MEDLINE    
Gallic acid and ellagic acid are two widely occurring phenolic compounds of plant origin, to which many biological activities including anticancer and antiviral activity have been attributed. A simple HPTLC method has been developed for the simultaneous quantification of gallic acid and ellagic acid. The method was validated for precision, repeatability, and accuracy. Instrumental precision was found to be 0.083 and 0.78, and the repeatability of the method was found to be 1.07 and 1.50 (% CV) for gallic acid and ellagic acid, respectively. The accuracy of the method was checked by a recovery study conducted at two different levels and the average percentage recovery was found to be 101.02% for gallic acid and 102.42% for ellagic acid. The above method was used for the quantification of gallic acid and ellagic acid content in seeds of Abrus precatorius Linn., whole plant of Phyllanthus maderaspatensis Linn., and flowers of Nymphaea alba Linn. The proposed HPTLC method for the simultaneous quantification of gallic acid and ellagic acid was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of herbal raw materials and for the quantification of these compounds in plant materials.
Milind Bagul; H Srinivasa; Harish Padh; M Rajani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  28     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-09     Completed Date:  2005-09-15     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  581-4     Citation Subset:  IM    
B. V. Patel Pharmaceutical Education and Research Development Centre, Thaltej-Gandhinagar highway, Thaltej, Ahmedabad - 380 054, India.
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MeSH Terms
Abrus / chemistry*
Chromatography, High Pressure Liquid / methods*
Densitometry / methods*
Ellagic Acid / analysis*
Gallic Acid / analysis*
Molecular Structure
Plants, Medicinal / chemistry
Reproducibility of Results
Seeds / chemistry*
Reg. No./Substance:
149-91-7/Gallic Acid; 476-66-4/Ellagic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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