Document Detail


A rapid antibody-based test for Sec-2, a marker for the short arm of chromosome 2 of rye (2RS).
MedLine Citation:
PMID:  18469949     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A simple monoclonal antibody-based screening test has been developed for the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three polypeptides known as Mr 75 000 gamma-secalins. Use of the antibody test for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 2BL-2RS translocations and 2R substitutions from 1BL-1RS translocations or nontranslocation wheats. Use of the antibody in analysis of segregating progeny for Sec-2 in several wheat backgrounds was successful. Results with a selection of the seed population were checked using protein gel electrophoresis, with 100% correct confirmation. Key words : rye, wheat, seed proteins, translocation, diagnostic test.
Authors:
J H Skerritt; R B Gupta; J L Andrews; F L Stoddard; N K Howes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  39     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  1996 Oct 
Date Detail:
Created Date:  2008-05-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1006-12     Citation Subset:  -    
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