| ran1+ controls the transition from mitotic division to meiosis in fission yeast. | |
| | |
MedLine Citation:
|
PMID: 3870979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have investigated the genetic and physiological control of meiosis in fission yeast. Nutritionally depleted h+/h- diploid cells become irreversibly commited to meiosis immediately prior to the initiation of premeiotic S phase. Premeiotic DNA synthesis requires matP+, matM+, mei2+ and mei3+ but not the mitotic cell cycle control gene, cdc2+. ran1+ is an essential gene, loss of which provokes sexual conjugation, premeiotic DNA synthesis, pseudo-meiosis and the sporulation of haploid cells. Our experiments suggest that sexual differentiation is achieved physiologically by the inhibition of ran1+ activity in a two-step process. In the first step, partial inhibition of ran1+ in starved haploid cells, leads to cell cycle arrest in G1 followed by sexual conjugation. In the second step, a pathway requiring the matP+, matM+ and mei3+ genes of the newly-formed zygote, further inhibits ran1+ and thereby commits the cell to meiosis. mei2+ is required for meiotic commitment after full inhibition of ran1+. ran1+ is normally essential for vegetative cell reproduction but is inessential in cells which have abnormally high levels of cAMP-dependent protein kinase. We propose that the ran1+ gene encodes a highly controlled protein kinase which shares key substrates with cAMP-dependent protein kinase. |
| | |
Authors:
|
D Beach; L Rodgers; J Gould |
Related Documents
:
|
18160409 - The cell cycle db: a systems biology approach to cell cycle analysis. 21327189 - Tracking cellular metabolomics in lipoapoptosis- and steatosis-developing liver cells. 16438919 - Exfoliative vaginal cytology during the oestrous cycle of west african dwarf goats. 12628359 - Cell-cycle dysregulation and anticancer therapy. 7929629 - The kinetics of h1 histone kinase activation during the cell cycle of wild-type and wee... 20144999 - The perennial organelle: assembly and disassembly of the primary cilium. 10863159 - In vivo dna damage in gastric epithelial cells. 17700069 - Cell cycle-and proteasome-dependent formation of etoposide-induced replication protein ... 2311409 - Hypothermic preservation of hepatocytes. ii. importance of ca2 and amino acids. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Current genetics Volume: 10 ISSN: 0172-8083 ISO Abbreviation: Curr. Genet. Publication Date: 1985 |
Date Detail:
|
Created Date: 1988-10-07 Completed Date: 1988-10-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8004904 Medline TA: Curr Genet Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 297-311 Citation Subset: IM |
Affiliation:
|
Cold Spring Harbor Laboratory, NY 11742. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alleles DNA Replication DNA, Fungal / genetics Genes, Fungal* Mutation Saccharomycetales / genetics* Schizosaccharomyces / genetics*, physiology |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Fungal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: On the nature and specificity of replicating instability in fission yeast.
Next Document: A geometric calculation of square and circular size equivalents for rectangular radiation fields in ...