Document Detail


A radioisotopic technique for analysis of free fatty acid reesterification in human adipose tissue.
MedLine Citation:
PMID:  3881046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reesterification rates of free fatty acids (FFA) formed by intracellular triglyceride hydrolysis in small fragments of human adipose tissue were measured. Subcutaneous gluteal adipose tissue, obtained by needle biopsy, was incubated in a buffered albumin medium containing [3H]palmitate and [14C]glucose, each of high specific activity. In triglycerides (TG) and diglycerides (DG) synthesized by the tissue, [14C]glucose is incorporated exclusively into the glyceride-glycerol moiety, and 3H appears solely in the esterified fatty acids. Since rates of TG and DG synthesis can be determined from 14C accumulation rates in these molecules, the total amounts of FFA esterified can also be calculated. The difference between this estimate of total FFA esterification and the moles of [3H]palmitate esterified to these molecules represents the amount of unlabeled FFA from ongoing TG hydrolysis that was reesterified during the incubation. FFA recycling by the reesterification pathway is an important mechanism for the control of the quantity and proportions of FFA and glycerol leaving the human adipocyte. Fasting and beta-adrenergic stimulation reduce the fraction of endogenously released FFA that are reesterified from resting values of 30-40% to 8-21%, thereby increasing the molar ratio of FFA to glycerol leaving the adipocyte. The technique described can be employed to monitor sequential changes in this important metabolic cycle in humans under a wide range of nutritional and clinical circumstances.
Authors:
R L Leibel; J Hirsch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  248     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-02-21     Completed Date:  1985-02-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E140-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism*
Carbon Radioisotopes / diagnostic use
Cell Membrane / metabolism
Fatty Acids, Nonesterified / metabolism*
Glycerides / biosynthesis*
Glycerol / analysis
Humans
Kinetics
Palmitic Acid
Palmitic Acids / metabolism*
Radioisotope Dilution Technique
Tritium / diagnostic use
Grant Support
ID/Acronym/Agency:
AM-30583/AM/NIADDK NIH HHS; GCRC-RR-00102/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids, Nonesterified; 0/Glycerides; 0/Palmitic Acids; 10028-17-8/Tritium; 56-81-5/Glycerol; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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