| A radioimmunoassay for the histamine H2-antagonist, tiotidine. | |
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MedLine Citation:
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PMID: 6151804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A specific and sensitive radioimmunoassay (RIA) for the histamine H2-antagonist, tiotidine, has been developed. The assay is based upon competition of tiotidine with [3H]-tiotidine for antibody (Ab) obtained from immunized rabbits. The immunogen used was a glutaraldehyde coupled conjugate of the tiotidine derivative (ICI 147,655) and bovine serum albumin (BSA). Displacement of [3H]-tiotidine by unlabeled tiotidine was competitive over a concentration range of 10-1000 fmol. The Ab was 100-fold less sensitive to the pharmacologically inactive metabolite of tiotidine (ICI 129,585) and did not cross-react with histamine, cimetidine or phenylguanidine. In dogs given an i.v dose of tiotidine, plasma levels of the antagonist, as measured by the RIA, were correlated with inhibition of histamine-induced gastric acid secretion. Tiotidine (0.3 or 0.6 mumol kg-1) caused a dose-dependent and transient decrease in acid secretion at plasma concentrations of 10(-6) to 10(-8) M. Other potential analytical and research uses of H2-antagonist radioimmunoassays are discussed. |
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Authors:
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P Dobson; T O Yellin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Agents and actions Volume: 15 ISSN: 0065-4299 ISO Abbreviation: Agents Actions Publication Date: 1984 Oct |
Date Detail:
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Created Date: 1985-03-05 Completed Date: 1985-03-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0213341 Medline TA: Agents Actions Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 162-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cimetidine / analogs & derivatives*, blood, pharmacology Dogs Gastric Acid / secretion Histamine / pharmacology Histamine H2 Antagonists / blood*, pharmacology Radioimmunoassay / methods |
| Chemical | |
Reg. No./Substance:
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0/Histamine H2 Antagonists; 51-45-6/Histamine; 51481-61-9/Cimetidine; 69014-14-8/tiotidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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