Document Detail


A radioimmunoassay for the histamine H2-antagonist, tiotidine.
MedLine Citation:
PMID:  6151804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A specific and sensitive radioimmunoassay (RIA) for the histamine H2-antagonist, tiotidine, has been developed. The assay is based upon competition of tiotidine with [3H]-tiotidine for antibody (Ab) obtained from immunized rabbits. The immunogen used was a glutaraldehyde coupled conjugate of the tiotidine derivative (ICI 147,655) and bovine serum albumin (BSA). Displacement of [3H]-tiotidine by unlabeled tiotidine was competitive over a concentration range of 10-1000 fmol. The Ab was 100-fold less sensitive to the pharmacologically inactive metabolite of tiotidine (ICI 129,585) and did not cross-react with histamine, cimetidine or phenylguanidine. In dogs given an i.v dose of tiotidine, plasma levels of the antagonist, as measured by the RIA, were correlated with inhibition of histamine-induced gastric acid secretion. Tiotidine (0.3 or 0.6 mumol kg-1) caused a dose-dependent and transient decrease in acid secretion at plasma concentrations of 10(-6) to 10(-8) M. Other potential analytical and research uses of H2-antagonist radioimmunoassays are discussed.
Authors:
P Dobson; T O Yellin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Agents and actions     Volume:  15     ISSN:  0065-4299     ISO Abbreviation:  Agents Actions     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-03-05     Completed Date:  1985-03-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0213341     Medline TA:  Agents Actions     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  162-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cimetidine / analogs & derivatives*,  blood,  pharmacology
Dogs
Gastric Acid / secretion
Histamine / pharmacology
Histamine H2 Antagonists / blood*,  pharmacology
Radioimmunoassay / methods
Chemical
Reg. No./Substance:
0/Histamine H2 Antagonists; 51-45-6/Histamine; 51481-61-9/Cimetidine; 69014-14-8/tiotidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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