Document Detail


A radioimmunoassay for the detection of adrenal autoantibodies.
MedLine Citation:
PMID:  3519015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes (100 micrograms/ml) were incubated with human sera diluted from 1:50 to 1:5000 and the retained antibodies detected by subsequent incubation with 125I-labelled protein A. The method was precise over the range of serum dilution of 1:250 to 1:5000. In the group of 30 patients with Addison's disease 19 had positive results in adrenal antibody radioimmunoassay (RIA). Comparative studies of RIA and immunofluorescence (IFL) revealed that there was partial correlation of adrenal antibody results in patients with high titre antibodies whereas RIA usually was more sensitive than IFL in patients with low titre antibodies. Computerized tomography (CT) displayed bilateral adrenal atrophy in most patients who had adrenal antibodies. On the other hand, patients with low RIA results and negative IFL antibodies had predominantly adrenal calcifications on scans.
Authors:
J Kosowicz; M Gryczynska; G F Bottazzo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  63     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-06-30     Completed Date:  1986-06-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  671-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Addison Disease / immunology*
Adrenal Glands / immunology*
Adult
Autoantibodies / analysis*
Female
Fluorescent Antibody Technique
Humans
Iodine Radioisotopes / diagnostic use
Male
Microsomes / immunology
Middle Aged
Polyethylene Glycols
Radioimmunoassay / methods*
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Iodine Radioisotopes; 0/Polyethylene Glycols
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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