| A radioimmunoassay for the detection of adrenal autoantibodies. | |
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MedLine Citation:
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PMID: 3519015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes (100 micrograms/ml) were incubated with human sera diluted from 1:50 to 1:5000 and the retained antibodies detected by subsequent incubation with 125I-labelled protein A. The method was precise over the range of serum dilution of 1:250 to 1:5000. In the group of 30 patients with Addison's disease 19 had positive results in adrenal antibody radioimmunoassay (RIA). Comparative studies of RIA and immunofluorescence (IFL) revealed that there was partial correlation of adrenal antibody results in patients with high titre antibodies whereas RIA usually was more sensitive than IFL in patients with low titre antibodies. Computerized tomography (CT) displayed bilateral adrenal atrophy in most patients who had adrenal antibodies. On the other hand, patients with low RIA results and negative IFL antibodies had predominantly adrenal calcifications on scans. |
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Authors:
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J Kosowicz; M Gryczynska; G F Bottazzo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 63 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-06-30 Completed Date: 1986-06-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 671-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Addison Disease
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immunology* Adrenal Glands / immunology* Adult Autoantibodies / analysis* Female Fluorescent Antibody Technique Humans Iodine Radioisotopes / diagnostic use Male Microsomes / immunology Middle Aged Polyethylene Glycols Radioimmunoassay / methods* |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Iodine Radioisotopes; 0/Polyethylene Glycols |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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