Document Detail

rDNA ITS sequence of Rhizopus oryzae: its application to classification and identification of lactic acid producers.
MedLine Citation:
PMID:  12951506     Owner:  NLM     Status:  MEDLINE    
Rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. To date, there were no easy methods to classify strains according to their acid production. The sequences of the ribosomal RNA-encoding DNA (rDNA) internal transcribed spacer (ITS) region of 64 strains of R. oryzae were analyzed and found to conserve mutations correspond to acid production. We have devised a way to use these mutations for a novel method to identify lactic-acid-producing Rhizopus oryzae, by designing specific polymerase chain reaction (PCR) primers on them. Touch down PCR using these primers amplified the ITS DNA of lactic acid producers specifically. By this method, we could isolate lactic acid producing strains from Indonesian fermented foods.
Ayumi Abe; Teruo Sone; I Nengah Sujaya; Katsuichi Saito; Yuji Oda; Kozo Asano; Fusao Tomita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-02     Completed Date:  2004-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1725-31     Citation Subset:  IM    
Laboratory of Applied Microbiology, the Graduate School of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
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MeSH Terms
Base Sequence
Biotechnology / methods
DNA, Fungal / genetics
DNA, Ribosomal Spacer / genetics*
Genes, Fungal / genetics
Lactic Acid / biosynthesis*
Molecular Sequence Data
Polymerase Chain Reaction / methods
Rhizopus / classification*,  genetics*,  metabolism
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Ribosomal Spacer; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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