Document Detail


A quick test for the simultaneous determination of intactness and concentration of spermatozoa.
MedLine Citation:
PMID:  6892079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fluorescence of ethidium bromide bound to the DNA of bull spermatozoa can be used for the estimation of intactness and concentration of spermatozoa of washed sperm samples. For the full permeability of the spermatozoal membrane for ethidium bromide digitonin is used in a concentration of 50 micrograms/ml. The fluorescence signals before and after digitonin treatment reflect the portion of cells with intact cellular membrane in the sample. The signal after addition of digitonin correlates to a high degree with the sperm concentration. The method exhibits a sufficient accuracy for the estimation of sperm quality. That applies for the reproducibility with individual ejaculates as well as for the correlation to other methods determining the intactness and sperm concentration. This simple single-step technique requires only about 2 X 10(7) spermatozoa corresponding to 10 to 20 microliters of an average bull ejaculate.
Authors:
W Halangk; R Bohnensack
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biologica et medica Germanica     Volume:  41     ISSN:  0001-5318     ISO Abbreviation:  Acta Biol. Med. Ger.     Publication Date:  1982  
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370276     Medline TA:  Acta Biol Med Ger     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  899-905     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Ethidium / diagnostic use*
Fluorescence
Male
Sperm Count
Spermatozoa* / physiology
Chemical
Reg. No./Substance:
3546-21-2/Ethidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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