Document Detail


A quick enzyme squash technique for detailed studies on female meiosis in Solanum.
MedLine Citation:
PMID:  3303456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple enzyme squash technique that enables detailed studies of meiosis in potato ovules has been developed. Fixation of ovules in iron-propionic-ethanol followed by enzymatic maceration and squashing in acetocarmine yielded numerous well preserved megasporocytes with nicely spread chromosomes. Resolution was sufficient, allowing detailed analysis of chromosome pairing and chiasma formation and readily permitting distinction between normal and desynaptic mutant plants. Whereas the use of previously developed ovule squash techniques has been restricted to cytogenetic analyses of plant species with relatively large megasporocytes and large chromosomes, the present technique is potentially more useful for analyses of species with small megasporocytes and small chromosomes.
Authors:
E Jongedijk
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  62     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-08-28     Completed Date:  1987-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  135-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cellulase
Chromosomes / ultrastructure*
Cytological Techniques*
Meiosis*
Plants / genetics,  ultrastructure*
Polygalacturonase
Solanum tuberosum
Chemical
Reg. No./Substance:
EC 3.2.1.15/Polygalacturonase; EC 3.2.1.4/Cellulase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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