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The quest for an optimized protocol for whole heart decellularization: A comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities.
MedLine Citation:
PMID:  21548726     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Decellularized cardiac ECM has been introduced as a template for cardiac tissue engineering, providing the advantages of a prevascularized scaffold that mimicks native micro- and macroarchitecture to a degree difficult to achieve with synthetic materials. Nonetheless, the decellularization protocols used to create acellular myocardial scaffolds vary widely throughout the literature. In this study we performed a direct comparison of three previously described protocols while introducing and evaluating a novel, specifically developed fourth protocol, by decellularizing whole rat hearts through software-controlled automatic coronary perfusion. Although all protocols preserved the macroarchitecture of the hearts and all resulting scaffolds could successfully be reseeded with myocytes, assessing their biocompatibility for 3D in vitro studies, we found striking differences concerning the microcomposition of the ECM-scaffolds on a histological and biochemical level. While laminin could still be detected in all groups, other crucial ECM-components, like elastin and collagen IV, were completely removed by at least one of the protocols. Further, only three protocols maintained a GAG-content comparable to native tissue, while the remaining DNA-content within the ECM varied highly throughout all four tested protocols. This study showed that the degree of acellularity and resulting ECM composition of decellularized myocardial scaffolds strongly differ depending on the decellularization protocol.
Authors:
Payam Akhyari; Hug Aubin; Patricia Gwanmesia; Mareike Barth; Stefanie Hoffmann; Joern Huelsmann; Karl-Heinz Preuss; Artur Lichtenberg
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-6
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Düsseldorf University Hospital, Department of Cardiovascular Surgery, Moorenstr. 5, Duesseldorf, Germany, 40225, +49-211-8118331, +49-211-8118333; payam.akhyari@med.uni-duesseldorf.de.
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