| The quaternary structure, antigenicity, and aggregational behavior of the secretory core protein of human hepatitis B virus are determined by its signal sequence. | |
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MedLine Citation:
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PMID: 1942254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human hepatitis B virus encodes a secretory core protein, referred to as the HBe protein, whose secretion is mediated by the pre-C signal sequence. Here we examined whether this sequence is important only for translocation of the HBe precursor (the precore protein) or whether it also contributes to the structural and biophysical properties of the mature HBe protein. When a truncated hepatitis B virus precore protein, lacking the basic C-terminal domain which is cleaved from the wild-type protein during its conversion into HBe, was expressed in human hepatoma cells, only a small amount of HBe-like protein was produced. This protein was slightly smaller than the wild-type HBe protein, suggesting that C-terminal cleavage of the precore protein does not occur at the suggested site. When the authentic signal sequence of the precore protein (the pre-C sequence) was replaced by the unrelated signal sequence of an influenza virus hemagglutinin, not only the full-length but also the C-terminally truncated protein was expressed and secreted with high efficiency. Western blot (immunoblot) analyses with nonreducing gels and conformation-specific monoclonal antibodies revealed that the HBe protein secreted under control of the pre-C signal sequence was a monomer with HBe antigenicity, whereas the HBe-like protein secreted under control of the hemagglutinin signal sequence was a disulfide-bridge-linked dimer with both HBe and HBc antigenicity. Electron microscopic examination of gradient-purified particulate core gene products showed that HBe protein secreted under control of the hemagglutinin signal sequence forms core particles, whereas HBe protein secreted under control of the pre-C sequence does not. Thus, the pre-C sequence not only mediates the secretion but also determines the structural and aggregational properties of the HBe protein. |
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Authors:
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H J Schlicht; G Wasenauer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of virology Volume: 65 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1991-12-26 Completed Date: 1991-12-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6817-25 Citation Subset: IM |
Affiliation:
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Department of Virology, University of Ulm, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antibodies, Monoclonal Base Sequence Cell Line Codon / genetics Genes, Viral Hepatitis B Core Antigens / genetics Hepatitis B e Antigens / genetics*, immunology, metabolism Hepatitis B virus / genetics*, immunology, physiology Humans Macromolecular Substances Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Protein Sorting Signals / genetics*, immunology, metabolism Recombination, Genetic Vaccinia virus / genetics |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Codon; 0/Hepatitis B Core Antigens; 0/Hepatitis B e Antigens; 0/Macromolecular Substances; 0/Oligodeoxyribonucleotides; 0/Protein Sorting Signals |
| Comments/Corrections | |
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