Document Detail


A quantitative study of testicular germ cell populations in masculinized neomale common carp (Cyprinus carpio L.).
MedLine Citation:
PMID:  18299213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The main objective of the present study was to investigate the effects of 17 alpha-methyltestosterone treatment upon the testicular germ cells of gynogenetic masculinized neomale common carp (Cyprinus carpio L.) in comparison with diploid carp. Gynogenetic common carp progeny (mean body weight, BW, 2.6+/-0.3g; mean total length, 10.4+/-0.5 cm) were treated for a period of 40 days with 17 alpha-methyltestosterone (MT) at a dose of 100mg kg(-1). The oral administration of MT resulted in 61.5-100% of fish exhibiting male gonads. The masculinized neomales exhibited reduced (P<0.05) body weight (BW=22.9+/-0.8) but significantly increased (P<0.05) mean testis weight (2.1+/-0.3) and mean gonadosomatic index (GSI=9.5+/-0.2%) in comparison with fish not treated with MT (BW 54.8+/-1.3; GSI=0.61%). Furthermore, treatment with MT also resulted in an increased number of fish exhibiting abnormal gonads. However, neomales did not exhibit abnormalities in the development of sperm ducts. MT treatment significantly increased germ cell volume, nuclear diameter, nuclear volume and cyst volume (P<0.01 in all cases) in MT-treated fish compared to untreated fish. The area occupied by seminiferous tubules, the number of Sertoli cells and germ cells per cyst, and the number of Leydig cells were significantly (P<0.05) greater in fish treated with MT. The carp neomales exhibited approximately 20-60% more Sertoli cells per cyst (P<0.05). Leydig cell nuclear volume and Leydig cell individual volume were significantly reduced in MT-treated groups (P<0.05) compared with untreated groups. In conclusion, our study strongly suggests that the abnormal gonadal structure evident in masculinized neomales could be explained by a combination of MT-induced genetic (homozygosity) and anabolic effects (upon germ and somatic cells).
Authors:
M Hulak; M Psenicka; K Coward; O Linhart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-25
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  515-24     Citation Subset:  IM    
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic. hulak@vurh.jcu.cz
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps / physiology*
Cell Proliferation / drug effects
Female
Gonads / anatomy & histology,  drug effects*
Leydig Cells / cytology,  drug effects
Male
Methyltestosterone / pharmacology*
Sertoli Cells / cytology,  drug effects
Sex Reversal, Gonadal
Spermatozoa / cytology*,  drug effects*
Testis / anatomy & histology,  cytology*,  drug effects
Chemical
Reg. No./Substance:
58-18-4/Methyltestosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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