Document Detail


A quantitative method for morphometric analysis in neuronal cell culture: unbiased estimation of neuron area and number of branch points.
MedLine Citation:
PMID:  7791366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The morphology and fine structure of neurons in vivo as well as in vitro are influenced by a variety of cell-adhesion and extracellular matrix molecules and soluble growth factors. To examine the effects of such molecules, we have developed a new method for the quantitation of several parameters associated with the morphology of neurons in culture. Whereas methods which have been traditionally used to perform quantitative morphometric analysis of neurons in vitro are often time-consuming and subjective, the methods we describe provide a rapid, efficient, and unbiased approach to morphometric analysis of cultured neurons.
Authors:
R Ventimiglia; B E Jones; A Møller
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  57     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-07-25     Completed Date:  1995-07-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  63-6     Citation Subset:  IM    
Affiliation:
Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Count
Cell Differentiation / physiology
Cell Size
Cell Survival / physiology
Cells, Cultured
Extracellular Matrix Proteins / metabolism
Immunohistochemistry
Neostriatum / cytology,  metabolism
Neurons / metabolism,  ultrastructure*
Rats
gamma-Aminobutyric Acid / metabolism
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 56-12-2/gamma-Aminobutyric Acid

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