Document Detail


A quantitative method for the determination of cyclopropenoid fatty acids in cottonseed, cottonseed meal, and cottonseed oil (Gossypium hirsutum) by high-performance liquid chromatography.
MedLine Citation:
PMID:  17323967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyclopropenoid fatty acids (CPFAs), found in cottonseed, have been shown to have detrimental health effects to susceptible livestock. Previous quantitative analytical methods for the determination of CPFAs expressed these acids in terms of their relative abundance with respect to other fatty acids in the oil, necessitating the concurrent analysis of other fatty acids. The proposed analytical method describes the quantitation of three relevant CPFAs for cotton (malvalic acid, sterculic acid, and dihydrosterculic acid) in cottonseed in micrograms per gram fresh weight of sample. The method involves extraction of the oil, saponification, and derivatization of the free fatty acids with 2-bromoacetophenone to give the phenacyl esters. These esters are then separated by dual-column reverse-phase high-performance liquid chromatography and quantitated via external standards. This is the first method to include external calibration standards for CPFAs and, as such, is capable of direct quantification with no further data conversion required. CPFA data generated from the analysis of cottonseed, cottonseed meal, and cottonseed oil produced in the United States in 2002 are presented.
Authors:
Janet C Obert; Donald Hughes; Wendy R Sorenson; Melinda McCann; William P Ridley
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Publication Detail:
Type:  Journal Article     Date:  2007-02-27
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-14     Completed Date:  2007-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2062-7     Citation Subset:  IM    
Affiliation:
Monsanto Company, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid / methods*
Cottonseed Oil / chemistry*
Cyclopropanes / analysis*
Fatty Acids / analysis*
Fatty Acids, Monounsaturated / analysis*
Chemical
Reg. No./Substance:
0/Cottonseed Oil; 0/Cyclopropanes; 0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 503-05-9/malvalic acid; 5711-28-4/dihydrosterculic acid; 738-87-4/sterculic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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