Document Detail


The quantitative conversion of morphine 3-beta-D glucuronide to morphine using beta-glucuronidase obtained from Patella vulgata as compared to acid hydrolysis.
MedLine Citation:
PMID:  8371549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose of this study was to evaluate the quantitative conversion of M-3G to morphine in human urine using a thermally stable beta-glucuronidase isolated from Patella vulgata; to compare these findings with those obtained from acid hydrolysis; and to compare between-run imprecision for both hydrolysis methods. We found both enzyme and acid hydrolysis techniques to be efficient, giving 90.4% and 92.8% conversion of M-3G to morphine, respectively. Also, both methods were found to be reproducible. Over a 14 week period, 20 opiate confirmation batches were analyzed by each hydrolysis method; the coefficient of variation for morphine liberated from M-3G was 5.5% for enzyme hydrolysis and 2.7% for acid hydrolysis.
Authors:
T A Jennison; E Wozniak; G Nelson; F M Urry
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  17     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:    1993 Jul-Aug
Date Detail:
Created Date:  1993-10-14     Completed Date:  1993-10-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  208-10     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Utah School of Medicine, Salt Lake City 84108.
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MeSH Terms
Descriptor/Qualifier:
Animals
Glucuronidase / metabolism*
Humans
Hydrolysis
Mollusca / enzymology
Morphine / metabolism*
Morphine Derivatives / metabolism*
Chemical
Reg. No./Substance:
0/Morphine Derivatives; 20290-09-9/morphine-3-glucuronide; 57-27-2/Morphine; EC 3.2.1.31/Glucuronidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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