| A quantitative assessment of the use of 36Cl- distribution to measure plasma membrane potential in isolated hepatocytes. | |
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MedLine Citation:
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PMID: 2480814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The plasma membrane potential of isolated rat hepatocytes was clamped at different values between 0 and -68 mV by addition of valinomycin in the presence of different extracellular concentrations of K+, and measured by the distribution of 86Rb+ between cells and medium. 36Cl- distribution came to steady state in 10-15 min. This steady-state distribution was compared to the plasma membrane potential over a range of values. 36Cl- distribution provided an accurate measurement of plasma membrane potential between -4 and -40 mV. At higher potentials intracellular chloride concentration is less than 20% of the extracellular concentration and errors due to uncertainties in the measurement of intracellular volume and of the contamination of cell pellets by extracellular medium precluded accurate determination of membrane potential: thus in our experiments 36Cl- underestimated the plasma membrane potential at -68 mV by 8 mV. |
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Authors:
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C D Nobes; M D Brand |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 987 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1990-02-02 Completed Date: 1990-02-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 115-23 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Cambridge, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Cell Membrane / physiology* Chlorides / metabolism* Chlorine Female Indicators and Reagents Kinetics Liver / physiology* Membrane Potentials Onium Compounds / metabolism Potassium / pharmacology Radioisotopes Rats Rats, Inbred Strains Rhodamines Rubidium Radioisotopes / metabolism Staining and Labeling Trityl Compounds / metabolism Trypan Blue Valinomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Indicators and Reagents; 0/Onium Compounds; 0/Radioisotopes; 0/Rhodamines; 0/Rubidium Radioisotopes; 0/Trityl Compounds; 15912-74-0/triphenylmethylphosphonium; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate; 72-57-1/Trypan Blue; 7440-09-7/Potassium; 7782-50-5/Chlorine; 989-38-8/rhodamine 6G |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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