Document Detail

A quantitative assessment of the use of 36Cl- distribution to measure plasma membrane potential in isolated hepatocytes.
MedLine Citation:
PMID:  2480814     Owner:  NLM     Status:  MEDLINE    
The plasma membrane potential of isolated rat hepatocytes was clamped at different values between 0 and -68 mV by addition of valinomycin in the presence of different extracellular concentrations of K+, and measured by the distribution of 86Rb+ between cells and medium. 36Cl- distribution came to steady state in 10-15 min. This steady-state distribution was compared to the plasma membrane potential over a range of values. 36Cl- distribution provided an accurate measurement of plasma membrane potential between -4 and -40 mV. At higher potentials intracellular chloride concentration is less than 20% of the extracellular concentration and errors due to uncertainties in the measurement of intracellular volume and of the contamination of cell pellets by extracellular medium precluded accurate determination of membrane potential: thus in our experiments 36Cl- underestimated the plasma membrane potential at -68 mV by 8 mV.
C D Nobes; M D Brand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  987     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-02     Completed Date:  1990-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Department of Biochemistry, University of Cambridge, U.K.
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MeSH Terms
Adenosine Triphosphate / metabolism
Cell Membrane / physiology*
Chlorides / metabolism*
Indicators and Reagents
Liver / physiology*
Membrane Potentials
Onium Compounds / metabolism
Potassium / pharmacology
Rats, Inbred Strains
Rubidium Radioisotopes / metabolism
Staining and Labeling
Trityl Compounds / metabolism
Trypan Blue
Valinomycin / pharmacology
Reg. No./Substance:
0/Chlorides; 0/Indicators and Reagents; 0/Onium Compounds; 0/Radioisotopes; 0/Rhodamines; 0/Rubidium Radioisotopes; 0/Trityl Compounds; 15912-74-0/triphenylmethylphosphonium; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate; 72-57-1/Trypan Blue; 7440-09-7/Potassium; 7782-50-5/Chlorine; 989-38-8/rhodamine 6G

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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