Document Detail

A quantitative assay for concanavalin A- and Ricinus communis agglutinin-mediated agglutinations of rat ascites hepatoma cells. Relationship between concanavalin A binding and cell agglutination.
MedLine Citation:
PMID:  164953     Owner:  NLM     Status:  MEDLINE    
A simple quantitative assay method was developed for the agglutination of rat ascites hepatoma cells mediated by Concanavalin A or Ricinus communis agglutinin. This method was based on the principle that the turbidity of a cell suspension is proportional to the sum of the cross-sectional area of cells and aggregatesmas predicted by the theoretical consideration, the turbidity decreased when cells were aggregated and the decrease was a function of the average number of the cells in aggregates. The agglutinability of the cells, judged by this method, showed a maximum value at a certain concentration of the agglutinin. By further addition of the agglutinin, the agglutinability slightly decreased from the maximum. These phenomena were observed both for Concanavalin A and Ricinus communis agglutinin. The binding and the agglutination experiments using [3-H]concanavalin A revealed that the binding to approx;0% of the total receptors caused a maximal agglutination. This suggested that the receptors responsible for the agglutination constitute only a small part of the total receptors on the surface.
I Kaneko; H Hayatsu; T Ukita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  392     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-08-04     Completed Date:  1975-08-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  131-40     Citation Subset:  IM    
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MeSH Terms
Agglutination Tests*
Binding Sites, Antibody
Carcinoma, Hepatocellular / immunology*,  metabolism
Concanavalin A* / analysis,  metabolism
Lectins* / analysis
Liver Neoplasms
Neoplasms, Experimental / immunology,  metabolism
Plant Lectins
Plants, Toxic
Protein Binding
Time Factors
Reg. No./Substance:
0/Binding Sites, Antibody; 0/Lectins; 0/Plant Lectins; 11028-71-0/Concanavalin A

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