Document Detail


A quantitative approach to recording peristaltic activity from segments of rat small intestine in vivo.
MedLine Citation:
PMID:  15787946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed methods that allow correlation of propulsive reflexes of the intestine with measurements of intraluminal pressure, fluid movement and spatio-temporal maps of intestinal wall movements for the first time in vivo. A segment of jejunum was cannulated and set up in a Trendelenburg recording system while remaining connected to the vascular and nerve supply of the anaesthetized rat. The resting intraluminal pressure in intact intestine was 2-4 mmHg. Hydrostatic pressures of 2, 4, 8 and 16 mmHg were imposed. At a baseline pressure of 4 mmHg, propulsive waves generated pressures of 9 +/- 1 mmHg, that progressed oral to anal at 2-5 mm s(-1). Individual propulsive waves propelled 0.8 +/- 0.4 mL of fluid. The frequency of propulsive waves increased with pressure, but peristaltic efficiency (mL per contraction) decreased with pressure increase between 4 and 16 mmHg. Atropine, as a bolus, transiently blocked peristalsis, but caused maintained block when infused. Hexamethonium blocked propulsive contractions. Inhibition of nitrergic transmission converted regular peristalsis to non-propulsive contractions. These studies demonstrate the utility of an adapted Trendelenburg method for quantitative investigation of motility and pharmacology of enteric reflexes in vivo.
Authors:
G Bogeski; A D Shafton; P D Kitchener; D M Ferens; J B Furness
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  17     ISSN:  1350-1925     ISO Abbreviation:  Neurogastroenterol. Motil.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-24     Completed Date:  2005-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  England    
Other Details:
Languages:  eng     Pagination:  262-72     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, VIC 3010, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atropine / pharmacology
Enzyme Inhibitors / pharmacology
Ganglionic Blockers / pharmacology
Hexamethonium / pharmacology
Intestinal Mucosa / drug effects,  physiology
Intestine, Small / physiology*
Male
Muscarinic Antagonists / pharmacology
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / metabolism
Peristalsis / drug effects,  physiology*
Pressure
Rats
Videotape Recording
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Ganglionic Blockers; 0/Muscarinic Antagonists; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-55-8/Atropine; 60-26-4/Hexamethonium; EC 1.14.13.39/Nitric Oxide Synthase

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