| A quantitative approach to recording peristaltic activity from segments of rat small intestine in vivo. | |
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MedLine Citation:
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PMID: 15787946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed methods that allow correlation of propulsive reflexes of the intestine with measurements of intraluminal pressure, fluid movement and spatio-temporal maps of intestinal wall movements for the first time in vivo. A segment of jejunum was cannulated and set up in a Trendelenburg recording system while remaining connected to the vascular and nerve supply of the anaesthetized rat. The resting intraluminal pressure in intact intestine was 2-4 mmHg. Hydrostatic pressures of 2, 4, 8 and 16 mmHg were imposed. At a baseline pressure of 4 mmHg, propulsive waves generated pressures of 9 +/- 1 mmHg, that progressed oral to anal at 2-5 mm s(-1). Individual propulsive waves propelled 0.8 +/- 0.4 mL of fluid. The frequency of propulsive waves increased with pressure, but peristaltic efficiency (mL per contraction) decreased with pressure increase between 4 and 16 mmHg. Atropine, as a bolus, transiently blocked peristalsis, but caused maintained block when infused. Hexamethonium blocked propulsive contractions. Inhibition of nitrergic transmission converted regular peristalsis to non-propulsive contractions. These studies demonstrate the utility of an adapted Trendelenburg method for quantitative investigation of motility and pharmacology of enteric reflexes in vivo. |
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Authors:
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G Bogeski; A D Shafton; P D Kitchener; D M Ferens; J B Furness |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society Volume: 17 ISSN: 1350-1925 ISO Abbreviation: Neurogastroenterol. Motil. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-24 Completed Date: 2005-05-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9432572 Medline TA: Neurogastroenterol Motil Country: England |
Other Details:
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Languages: eng Pagination: 262-72 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology and Centre for Neuroscience, University of Melbourne, Parkville, VIC 3010, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atropine / pharmacology Enzyme Inhibitors / pharmacology Ganglionic Blockers / pharmacology Hexamethonium / pharmacology Intestinal Mucosa / drug effects, physiology Intestine, Small / physiology* Male Muscarinic Antagonists / pharmacology NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / metabolism Peristalsis / drug effects, physiology* Pressure Rats Videotape Recording |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Ganglionic Blockers; 0/Muscarinic Antagonists; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-55-8/Atropine; 60-26-4/Hexamethonium; EC 1.14.13.39/Nitric Oxide Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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