| The putative penicillin-binding proteins 1 and 2 are important for viability, growth and cell morphology of Brucella melitensis. | |
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MedLine Citation:
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PMID: 18809265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The penicillin-binding proteins (PBPs) are enzymes that regulate the assembly of the peptidoglycan layer of the bacterial cell wall. The genome of Brucella melitensis strain 16M possesses seven pbp genes: three in pbp-1 family (designated as 1A, 1B, and 1C); one in pbp-2 family; and three in pbp-6 family (designated as 6A, 6B, and 6C). We investigated the importance of pbp-1 and pbp-2 genes to viability, cell morphology and infectivity of B. melitensis. A recombinant B. melitensis strain (designated 16MDeltapbp1C) was generated by disrupting the pbp-1C of strain 16M by allelic exchange. This strain produced nearly 20% smaller colonies on trypticase soy agar plates, and grew slower in trypticase soy broth compared to the strain 16M. Electron microscopy revealed that strain 16M exhibited native cocco-bacillus morphology, while 16MDeltapbp1C possessed a spherical morphology. Strain 16MDeltapbp1C did not differ from strain 16M in terms of recovery from infected mouse macrophage cell line J774.1, or recovery from spleens of infected BALB/c mice, suggesting that pbp-1C is dispensable for intracellular persistence of B. melitensis. Expression of mRNA of fixR, the gene downstream of pbp-1C was similar between the strains 16M and 16MDeltapbp1C suggesting that disruption of pbp-1C did not induce any polar effects. Multiple attempts to mutate pbp-1A, pbp-1B, or pbp-2 genes failed, most probably because these genes are indispensable for viability of B. melitensis. Our findings suggest that pbp-1C regulates in vitro growth and cell morphology, whereas pbp-1A, pbp-1B, and pbp-2 are essential for viability of B. melitensis. |
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Authors:
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Aloka B Bandara; Gerhardt G Schurig; Nammalwar Sriranganathan; Rajeev Prasad; Stephen M Boyle |
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Publication Detail:
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Type: Journal Article Date: 2008-08-05 |
Journal Detail:
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Title: Veterinary microbiology Volume: 133 ISSN: 0378-1135 ISO Abbreviation: Vet. Microbiol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-09 Completed Date: 2009-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 387-93 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences & Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0910, USA. abandara@vt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brucella melitensis / cytology*, genetics, metabolism* Cell Line Gene Expression Regulation, Bacterial / physiology Macrophages Mice Mice, Inbred BALB C Penicillin-Binding Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Penicillin-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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