Document Detail


The putative oncoprotein Bcl-3 induces cyclin D1 to stimulate G(1) transition.
MedLine Citation:
PMID:  11713278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bcl-3 is a distinctive member of the IkappaB family of NF-kappaB inhibitors because it can function to coactivate transcription. A potential involvement of Bcl-3 in oncogenesis is highlighted by the fact that it was cloned due to its location at a breakpoint junction in some cases of human B-cell chronic lymphocytic leukemia and that it is highly expressed in human breast tumor tissue. To analyze the effects of Bcl-3 dysregulation in breast epithelial cells, we created stable immortalized human breast epithelial cell lines either expressing Bcl-3 or carrying the corresponding vector control plasmid. Analysis of the Bcl-3-expressing cells suggests that these cells have a shortened G(1) phase of the cell cycle as well as a significant increase in hyperphosphorylation of the retinoblastoma protein. Additionally, the cyclin D1 gene was found to be highly expressed in these cells. Upon further analysis, Bcl-3, acting as a coactivator with NF-kappaB p52 homodimers, was demonstrated to directly activate the cyclin D1 promoter through an NF-kappaB binding site. Therefore, our results demonstrate that dysregulated expression of Bcl-3 potentiates the G(1) transition of the cell cycle by stimulating the transcription of the cyclin D1 gene in human breast epithelial cells.
Authors:
S D Westerheide; M W Mayo; V Anest; J L Hanson; A S Baldwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  21     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-19     Completed Date:  2001-12-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8428-36     Citation Subset:  IM    
Affiliation:
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Binding Sites
Blotting, Northern
Blotting, Western
Breast / metabolism
COS Cells
Cell Cycle
Cell Division
Cell Line
Cell Nucleus / metabolism
Cell Separation
Cloning, Molecular
Cyclin D1 / metabolism*
Epithelial Cells / metabolism
Flow Cytometry
G1 Phase*
Gene Expression Regulation, Neoplastic
Humans
Leukemia, B-Cell / metabolism
Luciferases / metabolism
Mice
NF-kappa B / antagonists & inhibitors
Phosphorylation
Plasmids / metabolism
Promoter Regions, Genetic
Proto-Oncogene Proteins / metabolism*
Retinoblastoma Protein / metabolism
Transcription Factors
Transfection
Grant Support
ID/Acronym/Agency:
CA 73756/CA/NCI NIH HHS; CA 75080/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/NF-kappa B; 0/Proto-Oncogene Proteins; 0/Retinoblastoma Protein; 0/Transcription Factors; 0/proto-oncogene protein bcl-3; 136601-57-5/Cyclin D1; EC 1.13.12.-/Luciferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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