Document Detail

The putative human stem cell marker, Rex-1 (Zfp42): structural classification and expression in normal human epithelial and carcinoma cell cultures.
MedLine Citation:
PMID:  16865673     Owner:  NLM     Status:  MEDLINE    
Human Rex-1 (hRex-1) (also referred to as zinc-finger protein-42, Zfp42) encodes a zinc finger protein expression of which is believed to be characteristic of pluripotent stem cells. We have applied bioinformatics to classify the relationship of human, rat, and mouse REX1 proteins in the C2H2 family of zinc finger proteins and demonstrate that REX1 is a member of the YY1 sub-family of transcription factors, which includes the Drosophila pleiohomeotic (Pho) protein. We have generated a molecular model of the human REX1 zinc finger domains based on the crystal structure of the YY1 transcription factor. To date, expression of hRex-1 and its extensively studied mouse homolog mRex-1, has been reported only in embryonic and adult stem cells and in differentiated spermatocytes. In this study, reverse transcription-PCR and Western analysis were employed to assay for hRex-1 expression in cultured normal human epithelial cells and human carcinoma cell lines. Expression of hRex-1 mRNA was detected in normal human epidermal keratinocytes, normal prostate epithelial cells (PrEC), bronchial, and small airway lung epithelial cells. Other stem cell markers, such as Oct 4, DAB2, and cMyc were also detected in normal human epidermal keratinocyte cultures. Expression of hRex-1 was also detected in some human tumor cell lines including MDA-MB-468 mammary carcinoma, SCC-15 head and neck squamous cell carcinoma, and N-TERA2 human teratocarcinoma cells. Western analyses confirmed expression of the human REX1 (ZFP42) protein in MDA-MB-468 cells and normal human keratinocytes. This research has identified model human cell culture systems, in addition to embryonic stem (ES) cells, in which Rex-1 is expressed, and this should enable the characterization of REX1 functions in normal adult epithelial cells and tumorigenic stem cells.
Nigel P Mongan; Kisha M Martin; Lorraine J Gudas
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  45     ISSN:  0899-1987     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  887-900     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 Wiley-Liss, Inc.
Department of Pharmacology, Weill Medical College, Cornell University, New York, New York 10021, USA.
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MeSH Terms
Amino Acid Sequence
Biological Markers / analysis
Blotting, Western
Carcinoma / genetics,  metabolism*
Carrier Proteins / chemistry*,  classification,  metabolism*
Epithelial Cells / metabolism*
Kruppel-Like Transcription Factors
Models, Biological
Molecular Sequence Data
Nuclear Proteins / chemistry,  classification
Protein Conformation
RNA, Messenger / analysis,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, Protein
Stem Cells / metabolism
Tumor Cells, Cultured
YY1 Transcription Factor / chemistry,  classification
Zinc Fingers*
Grant Support
Reg. No./Substance:
0/BCL11A protein, human; 0/Biological Markers; 0/Carrier Proteins; 0/Kruppel-Like Transcription Factors; 0/Nuclear Proteins; 0/RNA, Messenger; 0/YY1 Transcription Factor; 0/ZFP42 protein, human

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