| A purified cellular extract accelerates the cell cycle in Physarum polycephalum. | |
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MedLine Citation:
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PMID: 2257887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasmodia of the myxomycete Physarum polycephalum (strain Cl) were collected at different times during the cell cycle and extracts were prepared from homogenates using a buffer optimized for microinjection into plasmodial veins. These extracts were injected into plasmodia during the first 3 h of the cell cycle. The time of the following mitosis was monitored and compared with that of the buffer-injected controls. Extracts of plasmodia homogenized 45 min before late telophase accelerated the onset of mitosis in the injected plasmodium up to 70 min, i.e., an advance of 10-14% compared to the 8- to 10-h cell cycle duration of the controls. The accelerating activity vanished completely after heating, freezing, or protease digestion, thus indicating the peptide nature of the active agent. Purification of the active compound by means of gel filtration revealed a molecular mass of about 2500 Da. The active portion of the extract was further fractionated by HPLC and the activity determined in a single peak. |
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Authors:
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U Hobohm; A Hildebrandt; L Rensing |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental cell research Volume: 191 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-01-29 Completed Date: 1991-01-29 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 332-6 Citation Subset: IM |
Affiliation:
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Dept. Biology, University of Bremen, West Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / drug effects, physiology Cell Extracts / administration & dosage, analysis, physiology* Chromatography, Gel Chromatography, High Pressure Liquid Microinjections Molecular Weight Peptides / analysis, physiology Physarum / analysis, cytology* |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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