Document Detail


The purMN genes of Rhizobium leguminosarum and a superficial link with siderophore production.
MedLine Citation:
PMID:  10659713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We isolated a mutant of R. leguminosarum initially on the basis of reduced production of the siderophore vicibactin on chrome azurol sulfonate (CAS)/agar indicator plates. The mutation was in the purMN operon and the mutant was shown to be an adenine auxotroph and defective for nodulation of peas. The siderophore defect appears to be trivial, being due to diminished growth of the auxotroph on agar-based minimal medium, which contains unknown contaminant(s) that allow it grow poorly. Transcriptional fusions showed that purMN was transcribed at relatively high levels in media containing purines. Expression was enhanced, approximately twofold, if purines were omitted.
Authors:
J B Stevens; N G de Luca; J E Beringer; J P Ringer; K H Yeoman; A W Johnston
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  13     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-03     Completed Date:  2000-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  228-31     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ243305
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / genetics
Genes, Bacterial*
Molecular Sequence Data
Mutation
Peas / microbiology
Peptides, Cyclic / biosynthesis
Plasmids / genetics
Purines / metabolism
Rhizobium leguminosarum / genetics*,  metabolism*
Siderophores / biosynthesis*
Chemical
Reg. No./Substance:
0/Peptides, Cyclic; 0/Purines; 0/Siderophores; 0/vicibactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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