Document Detail


pur4 mutations are lethal to the male, but not the female, gametophyte and affect sporophyte development in Arabidopsis.
MedLine Citation:
PMID:  18441219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purine metabolism is crucial in living cells and involves three complex pathways in plants: the de novo synthesis, the salvage, and the degradation pathways. The relative importance of each pathway in plant development and reproduction, however, is still unclear. We identified two T-DNA insertions in the Arabidopsis (Arabidopsis thaliana) PUR4 gene (At1g74260) that encodes formylglycinamidine ribonucleotide synthase (EC 6.3.5.3), the fourth enzyme in the de novo purine biosynthesis pathway. The mutated alleles were never transmitted through the pollen of heterozygous plants but could be inherited through the female gametophyte, indicating that de novo purine synthesis is specifically necessary for pollen development. Because the pur4 mutations were lethal to the male gametophyte, homozygous pur4 plants could not be obtained. However, the reproductive phenotype of hetererozygous plants carrying the pur4-2 mutated allele was more severe than that carrying the pur4-1 mutated allele, and pur4-2/+ plants showed slightly delayed early development. We showed that the pur4-2 allele produces an antisense transcript and that the amount of PUR4 mRNA is reduced in these plants. Transient expression of a translational fusion with the green fluorescent protein in Arabidopsis plantlets showed that the formylglycinamidine ribonucleotide synthase protein is dually targeted to chloroplast and mitochondria, suggesting that at least some steps of the de novo purine biosynthesis pathway can take place in both organelles in Arabidopsis, a dual location previously thought to be a peculiarity of ureide-forming tropical legumes.
Authors:
Richard Berthomé; Muriel Thomasset; Marion Maene; Nathalie Bourgeois; Nicole Froger; Françoise Budar
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Publication Detail:
Type:  Journal Article     Date:  2008-04-25
Journal Detail:
Title:  Plant physiology     Volume:  147     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-05     Completed Date:  2008-08-05     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  650-60     Citation Subset:  IM    
Affiliation:
Station de Génétique et d'Amélioration des Plantes, Institut Jean-Pierre Bourgin, INRA UR254, 78026 Versailles cedex, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU091297
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  growth & development*
Genes, Lethal*
Genes, Plant*
Molecular Sequence Data
Mutation*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/RNA, Messenger
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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