Document Detail

A pulmonary large cell carcinoma cell line expressing neuroendocrine cell markers and human chorionic gonadotropin alpha-subunit.
MedLine Citation:
PMID:  1705538     Owner:  NLM     Status:  MEDLINE    
A cell line producing the neuroendocrine cell surface antigen and human chorionic gonadotropin (hCG) alpha-subunit, designated as KTA7, was established from human large cell carcinoma using a serum-free medium, ACL-3. KTA7 continued to grow in the ACL-3 medium, showing the morphological characteristics of large cell undifferentiated carcinoma. The KTA7 cells reacted with antibodies such as 6H7 and MOC1 directed against the cell surface antigens and PGP9.5 directed against a cytoplasmic protein of neuroendocrine cells but did not possess either most epithelial markers other than low-molecular-weight keratin (Cytokeratin) or neuron-specific enolase. The KTA7 cells, by immunostaining with anti-hCG subunit antibodies, were shown to produce hCG alpha- but not beta-subunit. Northern blot analysis showed KTA7 RNA to synthesize hCG alpha-subunit mRNA but not that of the hCG beta-subunit. Thus, the hCG alpha-subunit alone was independently expressed in KTA7. Chromosome analysis showed loss of alleles of chromosome 3p and 17 in KTA7 cells but not loss of 13q. KTA7 was considered to be derived from large cell undifferentiated carcinoma with neuroendocrine differentiation (large cell neuroendocrine tumor) and thus may fine use in studies on the pathobiology of large cell-type neuroendocrine tumors since it expresses at the same time marker substances of neuroendocrine differentiation and the hCG alpha-subunit.
K Kasai; T Kameya; K Kadoya; C Wada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  82     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-04-10     Completed Date:  1991-04-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  12-8     Citation Subset:  IM    
Department of Pathology, School of Medicine, Kitasato University.
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MeSH Terms
Carcinoma, Non-Small-Cell Lung / chemistry*,  ultrastructure
Glycoprotein Hormones, alpha Subunit / analysis*
Lung Neoplasms / chemistry*,  ultrastructure
Microscopy, Electron
Molecular Weight
Tumor Cells, Cultured / chemistry
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Glycoprotein Hormones, alpha Subunit; 0/Tumor Markers, Biological

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